Le Dall M, Nicaud J, Tréton B Y, Gaillardin C M
Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, CBAI, Institut National Agronomique, F-78850 Thiverval-Grignon, France.
Curr Genet. 1996 Apr;29(5):446-56. doi: 10.1007/BF02221513.
We have isolated the 3-phosphoglycerate kinase (PGK) gene of the yeast Yarrowia lipolytica by probing a genomic library with a PCR fragment amplified with primers deduced from two highly conserved regions of various PGKs. It is a unique sequence encoding a polypeptide of 417 residues with extensive homology to other PGKs, especially to that of Aspergillus nidulans (76% identity). The expression of the Y. lipolytica PGK1 gene proved to be higher on gluconeogenic substrates than on glycolytic ones. Haploid strains harboring a disrupted allele were able to grow on mixtures of a gluconeogenic carbon source and of a glycolytic one, but required proline supplementation in the presence of glucose, and were inhibited by glycerol.
我们通过用根据多种3-磷酸甘油酸激酶（PGK）的两个高度保守区域推导的引物扩增得到的PCR片段探测基因组文库，分离出了解脂耶氏酵母的3-磷酸甘油酸激酶（PGK）基因。它是一个独特的序列，编码一个由417个残基组成的多肽，与其他PGK具有广泛的同源性，尤其是与构巢曲霉的PGK（76%的同一性）。解脂耶氏酵母PGK1基因在糖异生底物上的表达被证明高于在糖酵解底物上的表达。携带中断等位基因的单倍体菌株能够在糖异生碳源和糖酵解碳源的混合物上生长，但在有葡萄糖存在时需要补充脯氨酸，并且会被甘油抑制。
