Tuite M F, Dobson M J, Roberts N A, King R M, Burke D C, Kingsman S M, Kingsman A J
EMBO J. 1982;1(5):603-8. doi: 10.1002/j.1460-2075.1982.tb01215.x.
The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.
酵母磷酸甘油酸激酶基因（PGK）的5'调控区与人类α-干扰素的编码序列融合。然后，将这种PGK-干扰素融合体导入基于2μm高拷贝数质粒载体的酵母中。含有该质粒的菌株产生的PGK-α-干扰素融合蛋白占细胞蛋白的1%-2%，并且干扰素活性的表达受可发酵碳源可用性的调节。该系统每升分批培养物能够产生多达15毫克的人类α-干扰素。
