Tucková L, Zídek Z, Hanikýrová M, Cukrowska B, Tlaskalová-Hogenová H, Barot-Ciorbaru R
Department of Immunology, Czech Academy of Science, Prague.
Clin Exp Immunol. 1996 May;104(2):215-20. doi: 10.1046/j.1365-2249.1996.23730.x.
The Gram-positive bacterium, Nocardia opaca, is a source of substances with adjuvant effect, ability to stimulate macrophages and natural killer cells for enhanced cytotoxity and cytokine production and B lymphocytes for polyclonal immunoglobulin secretion. We determined the immunogenicity of isolated N. opaca fractions and prepared MoAbs against immunogenic water-soluble mitogen (NWSM). Two main proteins of molecular mass 15 and 56 kD were detected in western blot analysis and isolated by affinity chromatography using anti-NWSM MoAb B7/7. Both these isolated nocardial antigens were found to stimulate mouse peritoneal macrophage NOS. The effect of 5 micrograms NWSM was comparable to that of 5 micrograms lipopolysaccharide (LPS) or 20 U of interferon-gamma (IFN-gamma) added to cell cultures. The MoAb B7/7 decreased No2- production induced by NWSM or by isolated nocardial antigens, but did not significantly influence the production elicited by LPS or IFN-gamma. On the other hand, NOS activation by NWSM was not affected by anti-IFN-gamma MoAb. The possible independent pathway for IFN-gamma and NWSM macrophage activation is discussed.
革兰氏阳性菌——不透明诺卡氏菌，是具有佐剂效应的物质来源，能够刺激巨噬细胞和自然杀伤细胞以增强细胞毒性和细胞因子产生，并刺激B淋巴细胞分泌多克隆免疫球蛋白。我们测定了分离出的不透明诺卡氏菌组分的免疫原性，并制备了针对免疫原性水溶性丝裂原（NWSM）的单克隆抗体。在蛋白质印迹分析中检测到分子量为15和56 kDa的两种主要蛋白质，并使用抗NWSM单克隆抗体B7/7通过亲和色谱法进行分离。发现这两种分离出的诺卡氏菌抗原均能刺激小鼠腹腔巨噬细胞一氧化氮合酶（NOS）。5微克NWSM的作用与添加到细胞培养物中的5微克脂多糖（LPS）或20单位干扰素-γ（IFN-γ）的作用相当。单克隆抗体B7/7可降低由NWSM或分离出的诺卡氏菌抗原诱导的NO2-产生，但对由LPS或IFN-γ诱导的产生没有显著影响。另一方面，NWSM对NOS的激活不受抗IFN-γ单克隆抗体的影响。本文讨论了IFN-γ和NWSM激活巨噬细胞的可能独立途径。
