Matsiota-Bernard P, Hentati B, Pié S, Legakis N, Nauciel C, Avrameas S
Laboratoire de Microbiologie, Hôpital Raymond Poincaré, Garches, France.
Clin Exp Immunol. 1996 May;104(2):228-35. doi: 10.1046/j.1365-2249.1996.15724.x.
Various infections can precede or aggravate autoimmune diseases. Yet a beneficial effect of infection has also been described an various mechanisms have been postulated to explain this effect. The aim of this study was to examine the hypothesis that infection can have an immunoregulatory effect on the autoimmune process via the increased production of natural polyreactive antibodies. The effect of Salmonella typhimurium infection on the lupus-like disease of (NZB x NZW)F1 (B/W) mice was therefore studied. The effect of IgM and IgG preparations isolated from the serum of S. typhimurium-infected C57B1/6 and CBA mice on the autoimmune disease of B/W mice was also tested. C57B1/6 and CBA mice were chosen because they are respectively genetically susceptible and resistant to S. typhimurium infection and they differ in their antibody response during the early phase of infection. CBA mice can mount a specific anti-bacterium antibody response, whereas C57B1/6 mice present increased production of polyreactive antibodies. The infection effect was evaluated on several disease parameters, i.e. survival, incidence of high grade proteinuria and serum IgM and IgG antibody activity directed against a panel of autoantigens. Our main findings were: (i) infection of B/W mice with an attenuated strain of S. typhimurium delayed the course of the autoimmune disease when performed before the appearance of autoimmune symptoms; and (ii) IgM and IgG preparations from S. typhimurium-infected C57B1/6 mice had a similar effect whereas the IgM and IgG preparations from infected CBA mice, as well as from normal C57B1/6 and CBA mice, were ineffective. These results suggest that S. typhimurium infection can beneficially influence the development of the autoimmune disease of B/W mice. The immunoregulatory effect of the infection seems to be related at least partially, to the increase of a particular population of antibodies, the polyreactive antibodies.
多种感染可能先于自身免疫性疾病出现或使其加重。然而，感染的有益作用也已被描述，并且人们提出了各种机制来解释这种作用。本研究的目的是检验以下假设：感染可通过增加天然多反应性抗体的产生，对自身免疫过程产生免疫调节作用。因此，研究了鼠伤寒沙门氏菌感染对（NZB×NZW）F1（B/W）小鼠狼疮样疾病的影响。还测试了从鼠伤寒沙门氏菌感染的C57B1/6和CBA小鼠血清中分离出的IgM和IgG制剂对B/W小鼠自身免疫性疾病的影响。选择C57B1/6和CBA小鼠是因为它们分别对鼠伤寒沙门氏菌感染具有遗传易感性和抗性，并且它们在感染早期的抗体反应有所不同。CBA小鼠能够产生特异性抗细菌抗体反应，而C57B1/6小鼠的多反应性抗体产生增加。通过几个疾病参数评估感染效果，即存活率、重度蛋白尿的发生率以及针对一组自身抗原的血清IgM和IgG抗体活性。我们的主要发现是：（i）在自身免疫症状出现之前，用减毒的鼠伤寒沙门氏菌菌株感染B/W小鼠可延缓自身免疫性疾病的进程；（ii）来自鼠伤寒沙门氏菌感染的C57B1/6小鼠的IgM和IgG制剂具有类似作用，而来自感染的CBA小鼠以及正常C57B1/6和CBA小鼠的IgM和IgG制剂则无效。这些结果表明，鼠伤寒沙门氏菌感染可对B/W小鼠自身免疫性疾病的发展产生有益影响。感染的免疫调节作用似乎至少部分与特定抗体群体即多反应性抗体的增加有关。
