Meshkinpour H, Vaziri N D, Zhou X J, Erickson R, Liao S Y, Oveisi F, Bemanian S, Hollander D
Department of Medicine, University of California, Irvine, USA.
Dig Dis Sci. 1996 May;41(5):984-8. doi: 10.1007/BF02091541.
Effects of iron overload on intestinal function and structure are unknown and were, therefore, investigated. Sprague-Dawley rats were randomized into an iron-overloaded group, which received a single subcutaneous injection of 1.2 g/kg elemental iron-dextran complex, and placebo-treated pair-fed controls. Animals were studied after a 10-month observation period. Intestinal permeability was assessed by measuring the urinary excretion of lactulose, rhamnose, and mannitol after oral administration. In addition, tissue nonheme iron content was measured, and histologic examination and morphometric measurements were carried out. The chronic iron-overloaded group showed a significant increase in intestine tissue iron content and stainable iron in the submucosa and muscularis propria and adipose tissue of the small intestine and lamina propria and muscularis mucosa of the large intestine. There was a significant decrease in the crypt depths without discernible change in the intestine permeability to any of the markers used. In addition, the iron-overloaded animals showed a significant number of iron-laden cells, which primarily consisted of macrophages, fibroblasts, myocytes, and adipocytes. In contrast, no iron-laden cells were present in tissues obtained from the normal control group. Thus, chronic experimental iron overload in rats leads to significant morphologic, but no permeability, alterations of the alimentary tract.
铁过载对肠道功能和结构的影响尚不清楚，因此我们对此展开了研究。将Sprague-Dawley大鼠随机分为铁过载组和安慰剂处理的配对喂养对照组，铁过载组接受单次皮下注射1.2 g/kg元素铁-右旋糖酐复合物。在10个月的观察期后对动物进行研究。通过测量口服乳果糖、鼠李糖和甘露醇后的尿排泄量来评估肠道通透性。此外，测量组织非血红素铁含量，并进行组织学检查和形态计量学测量。慢性铁过载组小肠黏膜下层、固有肌层和脂肪组织以及大肠固有层和黏膜肌层中的肠道组织铁含量和可染色铁显著增加。隐窝深度显著降低，而对所使用的任何标志物的肠道通透性均无明显变化。此外，铁过载动物体内出现大量含铁细胞，主要由巨噬细胞、成纤维细胞、肌细胞和脂肪细胞组成。相比之下，正常对照组的组织中未出现含铁细胞。因此，大鼠慢性实验性铁过载会导致消化道出现显著的形态学改变，但不会影响通透性。
