Moshonov J, Orstavik D, Yamauchi S, Pettiette M, Trope M
Department of Endodontics, School of Dentistry, University of North Carolina at Chapel Hill, 27599-7450, USA.
Endod Dent Traumatol. 1995 Oct;11(5):220-4. doi: 10.1111/j.1600-9657.1995.tb00492.x.
The purpose of this study was to assess the efficacy of Nd:YAG laser irradiation in disinfecting the root canal system. Seventy-five teeth were uniformly instrumented and sterilized, and their root canals were infected for 60 min with an overnight culture of Enterococcus faecalis in Tryptic Soy Broth. The teeth were divided into 6 groups: 1--non-infected controls; 2--infected controls; 3--infected, laser treated; 4--infected, laser dummy (dye only); 5--infected, laser dummy (dye and air-water spray); 6--infected, NaOCl-treated (reference group). After treatment, the root canals were dried and dentinal shavings removed for a post-treatment culture. Quantitative analysis of bacteria surviving the various treatments were performed and the bacterial counts for each group were compared. SEM analysis of teeth split longitudinally was used to illustrate the effect of treatment on the smear layer and on surface bacteria. Nd:YAG laser irradiation (group 3) significantly reduced the number of bacteria while NaOCl irrigation (group 6) effectively disinfected the canals.
本研究的目的是评估钕钇铝石榴石（Nd:YAG）激光照射对根管系统的消毒效果。75颗牙齿被均匀预备并消毒，其根管用粪肠球菌在胰蛋白胨大豆肉汤中的过夜培养物感染60分钟。这些牙齿被分为6组：1组——未感染对照组；2组——感染对照组；3组——感染且接受激光治疗；4组——感染且接受激光模拟治疗（仅使用染料）；5组——感染且接受激光模拟治疗（使用染料和空气-水喷雾）；6组——感染且接受次氯酸钠（NaOCl）治疗（参照组）。治疗后，干燥根管并清除牙本质碎屑以进行治疗后培养。对经各种治疗后存活的细菌进行定量分析，并比较每组的细菌计数。对纵向劈开的牙齿进行扫描电子显微镜（SEM）分析，以说明治疗对玷污层和表面细菌的影响。Nd:YAG激光照射（3组）显著减少了细菌数量，而NaOCl冲洗（6组）有效地对根管进行了消毒。
