Bouhidel K, Irish V F
Department of Biology, Yale University, New Haven, Connecticut 06520-8104, USA.
Dev Biol. 1996 Feb 25;174(1):22-31. doi: 10.1006/dbio.1996.0048.
Flowers develop from the coordinated division and differentiation of cells derived from the shoot apical meristem. By inducing chromosomal deletions in individual shoot apical meristem cells, we have generated Arabidopsis plants that are genetically mosaic for the homeotic PISTILLATA gene. Flowers bearing wild-type PISTILLATA epidermal tissue and mutant pistillata internal tissues are phenotypically normal. Based on this non-cell-autonomy, we suggest that PISTILLATA controls the production of a substance involved in cell-cell communication between the outer and inner tissue layers of the flower. These mosaic flowers were also used to assess the relative contributions of meristematic cells to the developing floral organs. These observations indicate that meristematic cells have discrete but somewhat variable contributions to the Arabidopsis flower. We have used these results to construct a fate map of the Arabidopsis floral primordium.
花由源自茎尖分生组织的细胞的协调分裂和分化发育而来。通过诱导单个茎尖分生组织细胞中的染色体缺失，我们培育出了在同源异型PISTILLATA基因上具有遗传镶嵌性的拟南芥植株。带有野生型PISTILLATA表皮组织和突变型PISTILLATA内部组织的花在表型上是正常的。基于这种非细胞自主性，我们认为PISTILLATA控制着一种参与花的外层和内层组织间细胞-细胞通讯的物质的产生。这些镶嵌花还被用于评估分生组织细胞对发育中的花器官的相对贡献。这些观察结果表明，分生组织细胞对拟南芥花的贡献是离散的，但略有变化。我们利用这些结果构建了拟南芥花原基的命运图谱。
