编码来自伯氏疏螺旋体菌株B31假定主要σ因子的基因序列。

Sequence of a gene encoding a putative primary sigma factor from Borrelia burgdorferi strain B31.

作者信息

Tsai C P, Pan M J

机构信息

Department of Veterinary Medicine, National Taiwan University, Taipei, Republic of China.

出版信息

Gene. 1996 Feb 2;168(1):123-4. doi: 10.1016/0378-1119(95)00760-1.

DOI:10.1016/0378-1119(95)00760-1

PMID:8626058

Abstract

Utilizing a polymerase chain reaction-based approach, the gene (rpoD) encoding the primary sigma factor from Borrelia burgdorferi strain B31 was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame (ORF) of 1632 bp (543 amino acids (aa), 63.7 kDa). Comparison with Escherichia coli sigma 70 and Bacillus subtilis sigma 43 showed a high degree of similarity in the aa sequences, especially for the regions that are known to be required for promoter recognition and core binding.

摘要

利用基于聚合酶链反应的方法，克隆并测序了编码伯氏疏螺旋体B31菌株主要σ因子的基因（rpoD）。核苷酸序列分析揭示了一个1632 bp的开放阅读框（ORF）（543个氨基酸（aa），63.7 kDa）。与大肠杆菌σ70和枯草芽孢杆菌σ43的比较显示，氨基酸序列具有高度相似性，特别是在已知对启动子识别和核心结合必需的区域。