Shiina T, Tanaka K, Takahashi H
Institute of Applied Microbiology, University of Tokyo, Japan.
Gene. 1991 Oct 30;107(1):145-8. doi: 10.1016/0378-1119(91)90308-x.
The complete nucleotide sequence of the hrdB gene, an essential gene of Streptomyces coelicolor A3(2), indicates the presence of an open reading frame encoding a putative polypeptide of 442 amino acid (aa) residues with an Mr of 48,412. The principal sigma-like transcriptional factor of S. coelicolor (HrdB) protein showed an extensive aa sequence homology with the known principal sigma factors of Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Myxococcus xanthus. The degree of sequence similarity between HrdB protein and the known principal sigma factors was distinct from that observed between the principal sigma factors and the alternative (minor) sigma factors. Essentially all of the functional domains proposed for the principal sigma factor of E. coli were conserved in HrdB protein. The putative sigma factor, HrdB, like that of B. subtilis had a short internal nonconserved region, which might be characteristic of Gram+ species.
天蓝色链霉菌A3(2)的必需基因hrdB的完整核苷酸序列表明，存在一个开放阅读框，其编码一个推定的由442个氨基酸残基组成的多肽，分子量为48412。天蓝色链霉菌的主要类σ转录因子（HrdB）蛋白与大肠杆菌、枯草芽孢杆菌、铜绿假单胞菌和粘球菌已知的主要σ因子显示出广泛的氨基酸序列同源性。HrdB蛋白与已知主要σ因子之间的序列相似程度不同于主要σ因子与替代（次要）σ因子之间观察到的相似程度。基本上，为大肠杆菌主要σ因子提出的所有功能结构域在HrdB蛋白中都是保守的。推定的σ因子HrdB与枯草芽孢杆菌的类似，有一个短的内部非保守区域，这可能是革兰氏阳性菌的特征。
