Solenberg P J, Cantwell C A, Tietz A J, McGilvray D, Queener S W, Baltz R H
Lilly Research Laboratories, ELi Lilly and Company, Indianapolis, IN 46285, USA.
Gene. 1996 Feb 2;168(1):67-72. doi: 10.1016/0378-1119(95)00765-2.
We explored transposition in Streptomyces fradiae (Sf) as a means to insert a second copy of the tylF gene to improve tylosin (Ty) production. Transposons Tn5096 and Tn5099 transposed relatively randomly in Sf, and many of the insertions caused no deleterious effects on Ty production yields. Tn5098, a derivative of Tn5096 containing tylF and tylJ genes, recombined into the chromosome into the tyl gene cluster and transposition was not observed. However, following the tagging of a neutral site (NS) by Tn5099 transposition, tylF was effectively inserted into the NS by homologous recombination (transposon exchange). Recombinants obtained by transposon exchange produced higher yields of Ty.
我们探索了弗氏链霉菌（Sf）中的转座作用，以此作为插入第二个tylF基因拷贝以提高泰乐菌素（Ty）产量的一种手段。转座子Tn5096和Tn5099在Sf中相对随机地转座，并且许多插入对Ty产量没有产生有害影响。Tn5098是包含tylF和tylJ基因的Tn5096的衍生物，它重组到染色体的tyl基因簇中，未观察到转座现象。然而，在通过Tn5099转座标记一个中性位点（NS）之后，tylF通过同源重组（转座子交换）有效地插入到NS中。通过转座子交换获得的重组体产生了更高产量的Ty。
