同义密码子选择控制大肠杆菌bla和ompA基因mRNA在体内的周转和数量。
Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genes.
作者信息
Deana A, Ehrlich R, Reiss C
机构信息
Laboratoire Structure et Dynamique du Génome, Centre de Génétique Moléculaire, Centre National de la Recherch Scientifique, Gif sur Yvette, France.
出版信息
J Bacteriol. 1996 May;178(9):2718-20. doi: 10.1128/jb.178.9.2718-2720.1996.
Abstract
A number of silent codon changes were made in two Escherichia coli genes. For the ompA gene, the replacement of seven consecutive frequently used codons with synonymous infrequently used codons reduced the ompA mRNA level and its half-life. For the bla gene, the exchange of 24 codons for the most frequently used synonymous codons extended the bla mRNA half-life. A modification of ribosome traffic could account for these observations.
摘要
在两个大肠杆菌基因中进行了一些沉默密码子的改变。对于ompA基因，用不常用的同义密码子替换七个连续常用密码子，降低了ompA mRNA水平及其半衰期。对于bla基因，将24个密码子替换为最常用的同义密码子，延长了bla mRNA的半衰期。核糖体移动的改变可以解释这些观察结果。
相似文献
1
Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genes.同义密码子选择控制大肠杆菌bla和ompA基因mRNA在体内的周转和数量。
J Bacteriol. 1996 May;178(9):2718-20. doi: 10.1128/jb.178.9.2718-2720.1996.
2
Silent mutations in the Escherichia coli ompA leader peptide region strongly affect transcription and translation in vivo.大肠杆菌ompA前导肽区域的沉默突变在体内强烈影响转录和翻译。
Nucleic Acids Res. 1998 Oct 15;26(20):4778-82. doi: 10.1093/nar/26.20.4778.
3
Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.翻译提前终止对mRNA稳定性的影响取决于核糖体释放的位点。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4890-4. doi: 10.1073/pnas.84.14.4890.
4
[Stability of messenger RNA of Escherichia coli ompA is affected by the use of synonymous codon].[大肠杆菌ompA信使核糖核酸的稳定性受同义密码子使用的影响]
C R Acad Sci III. 1993 Jul;316(7):628-32.
5
In vivo and in vitro identity of site specific cleavages in the 5' non-coding region of ompA and bla mRNA in Escherichia coli.大肠杆菌中ompA和bla mRNA 5'非编码区位点特异性切割的体内和体外特性
EMBO J. 1988 Jul;7(7):2269-75. doi: 10.1002/j.1460-2075.1988.tb03067.x.
6
The differential stability of the Escherichia coli ompA and bla mRNA at various growth rates is not correlated to the efficiency of translation.大肠杆菌ompA和bla mRNA在不同生长速率下的差异稳定性与翻译效率无关。
Gene. 1988 Dec 10;72(1-2):141-9. doi: 10.1016/0378-1119(88)90136-9.
7
Isolation of a mRNA instability sequence that is cis-dominant to the ompA stability determinant in Escherichia coli.在大肠杆菌中分离出一种对ompA稳定性决定因素呈顺式显性的mRNA不稳定序列。
Gene. 1996 Nov 14;179(2):263-70. doi: 10.1016/s0378-1119(96)00377-0.
8
Retarded RNA turnover in Escherichia coli: a means of maintaining gene expression during anaerobiosis.大肠杆菌中RNA周转延迟：一种在厌氧状态下维持基因表达的方式。
Mol Microbiol. 1993 Jul;9(2):375-81. doi: 10.1111/j.1365-2958.1993.tb01698.x.
9
The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency.ompA 5'非翻译RNA片段在大肠杆菌中作为一种生长速率调节的mRNA稳定剂发挥作用，其活性与翻译效率无关。
J Bacteriol. 1990 Aug;172(8):4472-81. doi: 10.1128/jb.172.8.4472-4481.1990.
10
Codon influence on protein expression in E. coli correlates with mRNA levels.密码子对大肠杆菌中蛋白质表达的影响与mRNA水平相关。
Nature. 2016 Jan 21;529(7586):358-363. doi: 10.1038/nature16509. Epub 2016 Jan 13.
引用本文的文献
1
Machine learning in computational biology to accelerate high-throughput protein expression.计算生物学中的机器学习以加速高通量蛋白质表达。
Bioinformatics. 2017 Aug 15;33(16):2487-2495. doi: 10.1093/bioinformatics/btx207.
2
The Yin and Yang of codon usage.密码子使用的阴阳观。
Hum Mol Genet. 2016 Oct 1;25(R2):R77-R85. doi: 10.1093/hmg/ddw207. Epub 2016 Jun 27.
3
Codon influence on protein expression in E. coli correlates with mRNA levels.密码子对大肠杆菌中蛋白质表达的影响与mRNA水平相关。
Nature. 2016 Jan 21;529(7586):358-363. doi: 10.1038/nature16509. Epub 2016 Jan 13.
4
The effects of codon context on in vivo translation speed.密码子上下文对体内翻译速度的影响。
PLoS Genet. 2014 Jun 5;10(6):e1004392. doi: 10.1371/journal.pgen.1004392. eCollection 2014 Jun.
5
Strong purifying selection at synonymous sites in D. melanogaster.在黑腹果蝇中，同义位点存在强烈的纯化选择。
PLoS Genet. 2013 May;9(5):e1003527. doi: 10.1371/journal.pgen.1003527. Epub 2013 May 30.
6
Quantifying the adaptive potential of an antibiotic resistance enzyme.量化抗生素耐药酶的适应潜力。
PLoS Genet. 2012 Jun;8(6):e1002783. doi: 10.1371/journal.pgen.1002783. Epub 2012 Jun 28.
7
The new pLAI (lux regulon based auto-inducible) expression system for recombinant protein production in Escherichia coli.新型 pLAI（lux 调控基因簇诱导型）表达系统在大肠杆菌中重组蛋白生产中的应用。
Microb Cell Fact. 2012 Jan 5;11:3. doi: 10.1186/1475-2859-11-3.
8
Single amino acid changes in the predicted RNase H domain of Escherichia coli RNase G lead to complementation of RNase E deletion mutants.大肠杆菌 RNase G 的预测 RNase H 结构域中的单个氨基酸变化导致 RNase E 缺失突变体的互补。
RNA. 2010 Jul;16(7):1371-85. doi: 10.1261/rna.2104810. Epub 2010 May 27.
9
RNA cleavage linked with ribosomal action.与核糖体作用相关的RNA切割
Genetics. 2005 Oct;171(2):419-25. doi: 10.1534/genetics.105.042515. Epub 2005 Jul 14.
10
A novel endoribonuclease, RNase LS, in Escherichia coli.大肠杆菌中一种新型核糖核酸内切酶RNase LS。
Genetics. 2005 Jan;169(1):13-20. doi: 10.1534/genetics.104.033290.
本文引用的文献
1
Transcription termination.转录终止。
Crit Rev Biochem Mol Biol. 1993;28(1):1-30. doi: 10.3109/10409239309082571.
2
The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation.大肠杆菌乳糖操纵子β-半乳糖苷酶（lacZ）信使核糖核酸（mRNA）的稳定性取决于其合成与翻译的同步性。
EMBO J. 1995 Jul 3;14(13):3252-61. doi: 10.1002/j.1460-2075.1995.tb07328.x.
3
Escherichia coli ribosomes translate in vivo with variable rate.大肠杆菌核糖体在体内以可变速率进行翻译。
EMBO J. 1984 Dec 1;3(12):2895-8. doi: 10.1002/j.1460-2075.1984.tb02227.x.
4
Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12: localization on hybrid plasmid pTU100 and expression of a fragment of the gene.大肠杆菌K-12完整外膜蛋白的克隆结构基因（ompA）：定位在杂交质粒pTU100上及该基因片段的表达
Mol Gen Genet. 1980;179(1):13-20. doi: 10.1007/BF00268440.
5
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.大肠杆菌中mRNA的衰变：转录本特定片段命运的研究
Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7. doi: 10.1073/pnas.80.3.653.
6
Translation is a non-uniform process. Effect of tRNA availability on the rate of elongation of nascent polypeptide chains.翻译是一个非均匀的过程。转运RNA可用性对新生多肽链延伸速率的影响。
J Mol Biol. 1984 Dec 15;180(3):549-76. doi: 10.1016/0022-2836(84)90027-5.
7
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
8
Mechanisms of mRNA decay in bacteria: a perspective.细菌中mRNA降解的机制：一个视角。
Gene. 1988 Dec 10;72(1-2):15-23. doi: 10.1016/0378-1119(88)90123-0.
9
E. coli RNases: making sense of alphabet soup.
Cell. 1985 Apr;40(4):731-2. doi: 10.1016/0092-8674(85)90330-7.
10
Codon contexts from weakly expressed genes reduce expression in vivo.弱表达基因的密码子上下文会降低体内表达。
J Mol Biol. 1989 Oct 5;209(3):359-78. doi: 10.1016/0022-2836(89)90003-x.
Zotero 插件