Sangameswaran L, Delgado S G, Fish L M, Koch B D, Jakeman L B, Stewart G R, Sze P, Hunter J C, Eglen R M, Herman R C
Institute of Pharmacology, Neurobiology Unit, Roche Bioscience, Palo Alto, CA 94304, USA.
J Biol Chem. 1996 Mar 15;271(11):5953-6. doi: 10.1074/jbc.271.11.5953.
Small neurons of the dorsal root ganglia (DRG) are known to play an important role in nociceptive mechanisms. These neurons express two types of sodium current, which differ in their inactivation kinetics and sensitivity to tetrodotoxin. Here, we report the cloning of the alpha-subunit of a novel, voltage-gated sodium channel (PN3) from rat DRG. Functional expression in Xenopus oocytes showed that PN3 is a voltage-gated sodium channel with a depolarized activation potential, slow inactivation kinetics, and resistance to high concentrations of tetrodotoxin. In situ hybridization to rat DRG indicated that PN3 is expressed primarily in small sensory neurons of the peripheral nervous system.
已知背根神经节（DRG）的小神经元在伤害性感受机制中起重要作用。这些神经元表达两种类型的钠电流，它们在失活动力学和对河豚毒素的敏感性方面有所不同。在此，我们报告了从大鼠DRG克隆出一种新型电压门控钠通道（PN3）的α亚基。在非洲爪蟾卵母细胞中的功能表达表明，PN3是一种电压门控钠通道，具有去极化激活电位、缓慢的失活动力学以及对高浓度河豚毒素的抗性。对大鼠DRG进行原位杂交表明，PN3主要在外周神经系统的小感觉神经元中表达。
