Gong D W, Bi S, Pratley R E, Weintraub B D
Molecular and Cellular Endocrinology Branch and Phoenix Epidemiology and Clinical Research Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Biol Chem. 1996 Feb 23;271(8):3971-4. doi: 10.1074/jbc.271.8.3971.
The human gene encoding the homolog of the mouse obese (ob) gene was isolated and partially characterized. The human ob gene consists of three exons and two introns and spans about 18 kilobase pairs (kb), encoding a 3.5-kb cDNA. A 3-kb 5'-flanking region of the gene was cloned and transient transfection assay with luciferase reporter confirmed the promoter activity in differentiated F442-A adipocytes. Potential regulatory elements are discussed in this report.
编码小鼠肥胖（ob）基因同源物的人类基因被分离并进行了部分特征分析。人类ob基因由三个外显子和两个内含子组成，跨度约为18千碱基对（kb），编码一个3.5 kb的cDNA。该基因的一个3 kb的5'侧翼区域被克隆，并且用荧光素酶报告基因进行的瞬时转染试验证实了其在分化的F442 - A脂肪细胞中的启动子活性。本报告讨论了潜在的调控元件。
