Venge J, Lampinen M, Håkansson L, Rak S, Venge P
Asthma Research Centre, University of Uppsala, Sweden.
J Allergy Clin Immunol. 1996 May;97(5):1110-5. doi: 10.1016/s0091-6749(96)70265-8.
The study was carried out to identify those molecules that are important in vivo in the attraction of eosinophil granulocytes to the lungs of patients with asthma. Asthmatic patients with birch pollen allergy had lavages performed before and during the pollen season, and the chemotactic activity of the bronchoalveolar lavage fluid was tested against normal eosinophils. The activity was significantly increased during the pollen season as compared with the activity before the pollen season (p less than 0.01). Neutralizing antibodies to IL-2, IL-5 and IL-8, leukemia inhibitory factor, and to RANTES were added to the bronchoalveolar lavage fluid. Antibodies to IL-5 and RANTES, but not to IL-2 and IL-8 or leukemia inhibitory factor, significantly inhibited the chemotactic activity for eosinophils (p less than 0.001). It is concluded that IL-5 and RANTES are important chemoattractants in the lungs of patients with allergic asthma. The effect of IL-5 may be that of a cofactor to the chemotactic molecules, of which RANTES may be one of the most important in allergic asthma.
本研究旨在确定在体内对嗜酸性粒细胞向哮喘患者肺部趋化起重要作用的分子。对桦树花粉过敏的哮喘患者在花粉季节前后进行支气管肺泡灌洗，并检测支气管肺泡灌洗液对正常嗜酸性粒细胞的趋化活性。与花粉季节前相比，花粉季节期间该活性显著增加（p<0.01）。将针对白细胞介素-2（IL-2）、IL-5、IL-8、白血病抑制因子以及调节激活正常T细胞表达和分泌因子（RANTES）的中和抗体加入支气管肺泡灌洗液中。针对IL-5和RANTES的抗体，而非针对IL-2、IL-8或白血病抑制因子的抗体，显著抑制了对嗜酸性粒细胞的趋化活性（p<0.001）。得出结论，IL-5和RANTES是过敏性哮喘患者肺部重要的趋化因子。IL-5的作用可能是作为趋化分子的辅助因子，其中RANTES可能是过敏性哮喘中最重要的趋化分子之一。
