Tokita H, Okamoto H, Iizuka H, Kishimoto J, Tsuda F, Lesmana L A, Miyakawa Y, Mayumi M
Immunology Division, Jichi Medical School, Tochigi-Ken, Japan.
J Gen Virol. 1996 Feb;77 ( Pt 2 ):293-301. doi: 10.1099/0022-1317-77-2-293.
Hepatitis C virus (HCV) isolates from 126 hepatitis patients in Jakarta, Indonesia were genotyped by PCR with genotype-specific primers deduced from the HCV core gene. Fifty-five isolates (44%) were classified as genotype II/1b, 15 (12%) as 1c, 33 (26%) as III/2a, and 1 (1%) as V/3a, while the remaining 22 (17%) were not classifiable into any of the five common genotypes (I/1a, II/1b, III/2a, IV/2b and V/3a) or 1c. Sequences of a part of the NS5b region [1093 bp (nucleotides 8279-9371)] of the 22 isolates of unclassifiable genotype were subjected to pair-wise comparison and phylogenetic analysis along with those of 62 isolates of 25 genotypes in nine genetic groups. Seven of the isolates were classified into 2e and two into 2f, representing novel genotypes in genetic group 2, while ten and three were classified into two new genetic groups, 10 and 11, respectively, and their genotypes were provisionally designated 10a and 11a. The isolates of genotype 10a (JK049) and 11a (JK046) were sequenced in full. Comparison of 24 HCV genomes including those of JK049 and JK046, over the entire genome and subgenomic regions, supported the classification of HCV into 11 genetic groups.
采用聚合酶链反应（PCR），利用从丙型肝炎病毒（HCV）核心基因推导的基因型特异性引物，对印度尼西亚雅加达126例肝炎患者的HCV分离株进行基因分型。55株分离株（44%）被归类为II/1b基因型，15株（12%）为1c基因型，33株（26%）为III/2a基因型，1株（1%）为V/3a基因型，其余22株（17%）无法归类为五种常见基因型（I/1a、II/1b、III/2a、IV/2b和V/3a）或1c基因型中的任何一种。对22株无法分型的分离株的NS5b区域部分序列[1093 bp（核苷酸8279 - 9371）]与九个基因群中25种基因型的62株分离株的序列进行成对比较和系统发育分析。其中7株分离株被归类为2e基因型，2株为2f基因型，代表基因群2中的新型基因型，而10株和3株分别被归类为两个新的基因群，即10和11，其基因型暂定为10a和11a。对10a基因型（JK049）和11a基因型（JK046）的分离株进行了全序列测定。包括JK049和JK046在内的24个HCV基因组在整个基因组和亚基因组区域的比较，支持将HCV分为11个基因群。
