Guangzhou Blood Center, Guangzhou, Guangdong, China.
Infect Genet Evol. 2013 Dec;20:225-9. doi: 10.1016/j.meegid.2013.08.014. Epub 2013 Sep 5.
In this study, we characterized the full-length genomes of 16 HCV isolates obtained from patients in a single hospital in China using overlapping PCR followed by DNA sequencing. The obtained genomes are 9414-9628 nucleotides in length, and each genome contains a single ORF of 9021-9102 nucleotides. Nine genomes represent the common subtypes 1a, 1b, 2a, 2b, 3a, 3b, and 6a, while seven represent the infrequent lineages 1c, 2f, 4d, and 5a, and two novel genotype 6 variants. GZ51969 and GZ52540 are subtype 1b isolates belonging to two unique clusters designated A and B, which account for 29.5% and 59.5% of the 1b infections in China, respectively. ZS542 and GZ98799 represent the first two complete genomes of the provisionally assigned subtype 2f. ZS96 and ZS202 are novel genotype 6 variants that may qualify for two new subtypes. ZS17, ZS537, and ZS631 represent three alien subtypes, namely, 1c, 4d, and 5a, which were detected in China for the first time in this study and may have been recently introduced as a result of globalization. Taken together, these results confirmed a large variety of HCV taxonomic lineages in China through the sequencing of their full-length genomes. These lineages represent six genotypes, 11 subtypes, and two novel variants. They were characterized for achieving a better understanding of the HCV genetic variation patterns and for possible future research applications.
在这项研究中，我们使用重叠 PCR 结合 DNA 测序的方法，对来自中国一家医院的 16 例 HCV 分离株的全长基因组进行了特征描述。获得的基因组长度为 9414-9628 个核苷酸，每个基因组包含一个长度为 9021-9102 个核苷酸的单一 ORF。其中 9 个基因组代表常见的亚型 1a、1b、2a、2b、3a、3b 和 6a，而另外 7 个代表罕见的亚型 1c、2f、4d 和 5a，以及两个新的 6 型变种。GZ51969 和 GZ52540 是属于两个独特簇 A 和 B 的 1b 亚型分离株，分别占中国 1b 感染的 29.5%和 59.5%。ZS542 和 GZ98799 代表了暂定分配的 2f 亚型的前两个完整基因组。ZS96 和 ZS202 是新型 6 型变种，可能符合两个新亚型的标准。ZS17、ZS537 和 ZS631 代表三种外来亚型，即 1c、4d 和 5a，这是在中国首次检测到，可能是由于全球化的影响而新近引入的。综上所述，这些结果通过对全长基因组的测序，证实了中国 HCV 分类群的多样性。这些分类群代表了六种基因型、11 个亚型和两个新变种。对它们进行了特征描述，以更好地了解 HCV 的遗传变异模式，并为可能的未来研究应用提供参考。
