Martelli F, Iacobini C, Caruso M, Felsani A
Istituto Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Rome, Italy.
J Virol. 1996 Mar;70(3):1433-8. doi: 10.1128/JVI.70.3.1433-1438.1996.
NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, delta, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.
NF-D是一种普遍存在的核因子，已被证明能特异性结合多瘤病毒调控区域中的一个DNA元件。在本报告中，我们证明NF-D与一种转录因子相同或非常相似，该转录因子有多种命名，如YY1、δ、NF-E1、UCRBP或CF1。此外，我们发现在多瘤病毒基因组中存在第二个DNA基序，位于第一个基序下游40 bp处，它能高亲和力结合YY1/NF-D。两个位点都位于主要晚期转录起始位点的下游。通过定点诱变，我们证明这两个元件可能通过协同机制对晚期启动子的活性有正向贡献。我们还证明，YY1/NF-D功能对晚期启动子活性的需求因细胞系而异。
