Zhang J, Temin H M
Department of Microbiology and Immunology, Markey Cancer Center, University of Kentucky, Lexington 40536, USA.
J Virol. 1996 Mar;70(3):2019-21. doi: 10.1128/JVI.70.3.2019-2021.1996.
Retroviruses, as a result of the presence of two identical RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination occurs, although the rate is only 0.1% of the rate of homologous recombination. We developed a protocol to study transduction of cellular proto-oncogenes in a single cycle of retrovirus replication. The psi sequences is a cis required sequence for packaging viral RNA into viral particles. To test if the rate of nonhomologous recombination increases about 1,000 times when the psi sequence is deleted, as reported by other, we modified vectors we used previously (J. Zhang and H. M. Temin, Science 259:234-238, 1993). Our results indicated that the recombination rate did not undergo the increase of about 1,000 times when the psi sequence of a chimeric RNA was deleted.
逆转录病毒由于其病毒粒子中存在两个相同的RNA分子，因而重组率很高。当二聚体RNA分子中存在非同源RNA时，就会发生非同源重组，尽管其发生率仅为同源重组率的0.1%。我们开发了一种方案来研究逆转录病毒复制单周期中细胞原癌基因的转导。ψ序列是将病毒RNA包装到病毒颗粒中的顺式必需序列。为了检验当ψ序列缺失时非同源重组率是否如其他人所报道的那样增加约1000倍，我们对之前使用的载体进行了改造（J. Zhang和H. M. Temin，《科学》259:234 - 238，1993）。我们的结果表明，当嵌合RNA的ψ序列缺失时，重组率并未增加约1000倍。
