Mikovits J A, Hoffman P M, Rethwilm A, Ruscetti F W
Biological Carcinogenesis Development Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
J Virol. 1996 May;70(5):2774-80. doi: 10.1128/JVI.70.5.2774-2780.1996.
The infectivity of human foamy virus (HFV) was examined in primary and cultured human leukocytes. Cell-free infectious viral stocks of HFV were prepared from the human kidney cell line 293 transfected with an infectious molecular clone of HFV. HFV productively infects a variety of human myeloid and lymphoid cell lines. In addition, primary cell cultures enriched for human CD4+, monocytes and brain-derived microglial cells, were readily infected by HFV. Interestingly, while infected primary CD4+ lymphocytes and microglial cells showed marked cytopathology characteristic of foamy virus, HFV-infected monocyte-derived macrophages failed to show any cytopathology. In addition, marked cytotoxicity due to HFV infection was seen in both human T-cell leukemia virus type 1- and human immunodeficiency virus type 1-infected T-cell lines and in human immunodeficiency virus type 1-infected monocytoid cell lines. Thus, HFV infection produces differential cytopathology in a wide host range of primary human leukocytes and hematopoietic cell lines.
在原代和培养的人白细胞中检测了人泡沫病毒（HFV）的感染性。从转染了HFV感染性分子克隆的人肾细胞系293制备无细胞感染性病毒储备液。HFV能有效感染多种人髓系和淋巴系细胞系。此外，富含人CD4⁺细胞、单核细胞和脑源性小胶质细胞的原代细胞培养物很容易被HFV感染。有趣的是，虽然感染的原代CD4⁺淋巴细胞和小胶质细胞表现出泡沫病毒特有的明显细胞病理学特征，但HFV感染的单核细胞衍生巨噬细胞未表现出任何细胞病理学特征。此外，在1型人T细胞白血病病毒和1型人免疫缺陷病毒感染的T细胞系以及1型人免疫缺陷病毒感染的单核细胞样细胞系中，均观察到HFV感染导致的明显细胞毒性。因此，HFV感染在广泛的原代人白细胞和造血细胞系宿主范围内产生不同的细胞病理学表现。
