植物类逆转录病毒水稻东格鲁杆状病毒翻译过程中依赖位置的ATT起始
Position-dependent ATT initiation during plant pararetrovirus rice tungro bacilliform virus translation.
作者信息
Fütterer J, Potrykus I, Bao Y, Li L, Burns T M, Hull R, Hohn T
机构信息
Institute for Plant Sciences, Zürich, Switzerland.
出版信息
J Virol. 1996 May;70(5):2999-3010. doi: 10.1128/JVI.70.5.2999-3010.1996.
Abstract
The expression of the rice tungro bacilliform virus open reading frame I was studied in transiently transfected protoplasts. Expression occurs despite the presence of a long leader sequence and the absence of a proper ATG initiation codon. Translation is initiated at an ATT codon. The efficiency of initiation in rice protoplasts depends strongly on the mechanism by which ribosomes reach this codon. From the effects of scanning-inhibiting structures inserted into different leader regions, it can be deduced that this mechanism is related to the ribosome shunt described for cauliflower mosaic virus 35S RNA. The process delivers initiation-competent ribosomes to the region downstream of the leader and is so precise that only the second of two potential start codons only 12 nucleotides apart is recognized. The ATT codon that is used when it is present downstream of the leader is hardly recognized as a start codon by ribosomes that reach it by scanning.
摘要
在瞬时转染的原生质体中研究了水稻东格鲁杆状病毒开放阅读框I的表达。尽管存在长的前导序列且没有合适的ATG起始密码子，但仍会发生表达。翻译起始于一个ATT密码子。水稻原生质体中的起始效率强烈依赖于核糖体到达该密码子的机制。从插入不同前导区域的扫描抑制结构的影响可以推断，该机制与花椰菜花叶病毒35S RNA所描述的核糖体跳跃有关。该过程将具有起始能力的核糖体传递到前导序列下游的区域，并且非常精确，以至于仅识别相距仅12个核苷酸的两个潜在起始密码子中的第二个。当它位于前导序列下游时使用的ATT密码子，对于通过扫描到达它的核糖体来说，几乎不被识别为起始密码子。
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