Gassner P, Adler I D
Institut für Säugetiergenetik, GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Neuherberg, Oberschleissheim, Germany.
Mutat Res. 1996 Apr 6;367(4):195-202. doi: 10.1016/s0165-1218(96)90077-4.
Monomeric acrylamide was tested for its potential to induce aneuploidy in spermatocytes and bone marrow cells of mice. For this purpose, chromosomes from metaphase spreads were counted semi-automatically. In both test systems, cell proliferation was monitored, determining the meiotic index of spermatocytes and the average generation time of bone marrow cells after BrdU incorporation, respectively. No indications could be seen for different sensitivity of somatic and germinal cells towards acrylamide. With a dose of 120 mg/kg, the chemical caused cell cycle delay in both germ line and somatic cells. There was diverging response with respect to the balance of hypo- and hyperploidy. While the percentage of chromosome loss was significantly elevated in both test systems, acrylamide treatment did not increase the frequency of hyperploid cells. Interpreting these results on the basis of conventional test protocols, acrylamide should not be considered as an aneugen. The conservative approach, however, may be inadequate for the detection of aneugenic mechanisms different from non-disjunction.
对单体丙烯酰胺诱导小鼠精母细胞和骨髓细胞非整倍体的可能性进行了测试。为此，对中期染色体铺展上的染色体进行了半自动计数。在两个测试系统中，监测细胞增殖，分别测定精母细胞的减数分裂指数和掺入BrdU后骨髓细胞的平均世代时间。未发现体细胞和生殖细胞对丙烯酰胺的敏感性存在差异。剂量为120mg/kg时，该化学物质导致生殖细胞系和体细胞的细胞周期延迟。在亚倍体和超倍体的平衡方面存在不同的反应。虽然在两个测试系统中染色体丢失的百分比均显著升高，但丙烯酰胺处理并未增加超倍体细胞的频率。根据传统测试方案解释这些结果，丙烯酰胺不应被视为一种非整倍体诱变剂。然而，这种保守方法可能不足以检测不同于不分离的非整倍体诱变机制。
