Wu F Y, Iijima K, Nishida A, Higurashi M
Department of Maternal and Child Health, Faculty of Medicine, University of Tokyo, Japan.
Mutat Res. 1996 Apr 6;367(4):261-4. doi: 10.1016/s0165-1218(96)90086-5.
This study determines whether irradiation by blue or green light has an adverse effect on the DNA of Down syndrome (DS) cells by examining the sister chromatid exchange (SCE) frequency of peripheral lymphocytes obtained from five neonates with DS and five karyotypically normal neonates (control). Lymphocytes in G0 of the cell cycle were irradiated with blue or green fluorescent light for 1, 2, 4 or 6 h, and then cultured using a conventional method. Our results revealed that the induction of SCEs per cell in both groups increased in a dose-dependent manner, although more SCEs were respectively induced by the blue light. In addition, after 6 h of blue light irradiation, the net-induced SCEs in the DS groups were higher than those in the control groups.
本研究通过检测5名唐氏综合征（DS）新生儿和5名核型正常新生儿（对照组）外周淋巴细胞的姐妹染色单体交换（SCE）频率，来确定蓝光或绿光照射是否会对DS细胞的DNA产生不利影响。将处于细胞周期G0期的淋巴细胞用蓝光或绿光照射1、2、4或6小时，然后采用常规方法进行培养。我们的结果显示，两组中每细胞的SCE诱导均呈剂量依赖性增加，尽管蓝光分别诱导出更多的SCE。此外，蓝光照射6小时后，DS组的净诱导SCE高于对照组。
