Sloop R R, Escutin R O, Matus J A, Cole B A, Peterson G W
Department of Neurology, Loma Linda University, CA 92354, USA.
Neurology. 1996 May;46(5):1382-6. doi: 10.1212/wnl.46.5.1382.
To develop a human model for measuring the effect of intramuscularly injected botulinum toxin, we injected both extensor digitorum brevis (EDB) muscles in 13 healthy volunteers with seven varying doses of botulinum toxin type A. We measured, pre- and postinjection, EDB M-wave amplitude, area, and mean rectified voltage (MRV) (obtained during maximal voluntary muscle activation). There was a logarithmic-appearing dose-response relationship between increasing doses of botulinum toxin and decline in EDB M-wave amplitude, area, and MRV. The decline was incrementally less at higher doses of toxin and appeared to level off at a maximal effect of 85 to 90% decrement from baseline (85 to 90% "paralysis") at 15 to 20 units. The peak toxin effect was present on day 6 postinjection. Measurement of EDB M-wave amplitude, area, and MRV is a simple objective method for quantifying the onset and degree of human muscle "paralysis" following botulinum-toxin injection.
为建立一个用于测量肌肉注射肉毒杆菌毒素效果的人体模型，我们向13名健康志愿者的双侧趾短伸肌（EDB）注射了七种不同剂量的A型肉毒杆菌毒素。在注射前和注射后，我们测量了EDB的M波振幅、面积和平均整流电压（MRV）（在最大自主肌肉激活期间获得）。随着肉毒杆菌毒素剂量的增加，EDB的M波振幅、面积和MRV呈对数剂量反应关系下降。在较高剂量的毒素下，下降幅度逐渐减小，在15至20单位时，最大效应似乎稳定在比基线降低85%至90%（85%至90%“麻痹”）。毒素的峰值效应出现在注射后第6天。测量EDB的M波振幅、面积和MRV是一种简单客观的方法，用于量化肉毒杆菌毒素注射后人体肌肉“麻痹”的起始和程度。
