Titoy G A
Helminthology Division, Onderstepoort Veterinary Institute, South Africa.
Onderstepoort J Vet Res. 1995 Sep;62(3):215.
A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37 degrees C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0 degree C in 82.8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69.1% as assessed by motility.
本文描述了一种用于冷冻保存厚皮盖氏线虫第三期幼虫的技术。该技术包括将带鞘感染性幼虫在37℃下于40%（v/v）乙二醇中孵育7分钟，然后在0℃下于82.8%（v/v）冷冻保护剂混合物中孵育2分钟，之后将幼虫转移至液氮中。通过该技术获得的存活率一直很高：通过活力评估为69.1%。
