An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm).

A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37 degrees C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0 degree C in 82.8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69.1% as assessed by motility.