Stevens J W, Palechek P L, Griebling T L, Midura R J, Rokhlin O W, Cohen M B
Department of Orthopaedic Surgery, University of Iowa, Iowa City, 52242-1009, USA.
Prostate. 1996 Mar;28(3):153-61. doi: 10.1002/(SICI)1097-0045(199603)28:3<153::AID-PROS2>3.0.CO;2-G.
We have examined the expression of the transmembrane glycoproteins CD44 in four human prostate tumor cell lines. Expression was examined at the protein level by flow cytometric analysis and Western blot, and at the mRNA level by reverse transcription-polymerase chain reaction (RT-PCR). All four cell lines (DU145, LNCaP, PC3, and ND1) expressed the standard CD44 isoform (CD44s) at the mRNA level and all cell lines except LNCaP expressed CD44s at the protein level. All four cell lines contained one or more isoforms containing the v6 region (exon 10) at the mRNA level, which has been associated with metastatic potential. However, a subpopulation of LNCaP and ND1 cells showed protein expression of v6. In addition, soluble CD44 isoforms were identified in cultured supernatants from all cell lines except LNCaP. These results show that CD44 isoforms are expressed on human prostate tumor cell lines, including the expression of variant isoforms containing the v6 region, and provide a rationale for the further study of this cellular adhesion molecule in prostate cancer. In addition, preliminary results indicate altered expression of CD44 in human prostatic adenocarcinomas examined immunohistochemically.
我们检测了四种人前列腺肿瘤细胞系中跨膜糖蛋白CD44的表达情况。通过流式细胞术分析和蛋白质印迹法在蛋白质水平检测表达，通过逆转录-聚合酶链反应（RT-PCR）在mRNA水平检测表达。所有四种细胞系（DU145、LNCaP、PC3和ND1）在mRNA水平均表达标准CD44异构体（CD44s），除LNCaP外的所有细胞系在蛋白质水平均表达CD44s。所有四种细胞系在mRNA水平均含有一种或多种包含v6区域（外显子10）的异构体，该区域与转移潜能相关。然而，LNCaP和ND1细胞的一个亚群显示v6的蛋白质表达。此外，在除LNCaP外的所有细胞系的培养上清液中均鉴定出可溶性CD44异构体。这些结果表明CD44异构体在人前列腺肿瘤细胞系上表达，包括含有v6区域的变异异构体的表达，并为进一步研究这种细胞粘附分子在前列腺癌中的作用提供了理论依据。此外，初步结果表明，通过免疫组织化学检测，人前列腺腺癌中CD44的表达发生了改变。
