Roth D A, Bradley J P, Levine J P, McMullen H F, McCarthy J G, Longaker M T
Institute of Reconstructive Plastic Surgery at New York University Medical Center, New York, USA.
Plast Reconstr Surg. 1996 Apr;97(4):693-9. doi: 10.1097/00006534-199604000-00001.
The biology underlying normal and premature cranial suture fusion remains unknown. The purpose of this study was to investigate the role of the dura mater in cranial suture fusion. In the Sprague Dawley rat model, the posterior frontal cranial suture fuses between 10 and 20 days of postnatal life. The effect of separating the posterior frontal cranial suture from its underlying dura mater with an intervening silastic sheet was studied. Sixty rat pups, age 8 days, were divided into four groups of 15. Group A served as unoperated controls. Group B, the experimental group, underwent craniotomy, dural elevation, and insertion of a silicone sheet between the posterior frontal cranial suture and the underlying dura. Two operative sham groups were included. Group C underwent craniotomy and dural deflection only. Group D underwent craniotomy alone without dural deflection. The rats were sacrificed at 15, 22, and 30 days of age. The results showed that the unoperated animals (group A) demonstrated normal initiation of suture fusion at 15 days and complete fusion by 22 days. Group B animals, with silicone sheet barriers placed, showed persistent patency of sutures at 22 days. Initiation of suture fusion was delayed until 30 days. Sham group C, animals with craniotomy and dural deflection, showed that initiation of fusion was delayed until 22 days with complete fusion by 30 days of age. Sham group D, craniotomy alone, had the same normal temporal sequence of suture fusion as the unoperated control group A. These data indicate that normal cranial suture fusion is delayed when the suture-dural interaction is interrupted by a surgically place barrier or by simple dural deflection. Furthermore, interaction between the dura and the overlying suture appears to direct suture fusion.
正常和过早颅骨缝融合的生物学机制尚不清楚。本研究的目的是探讨硬脑膜在颅骨缝融合中的作用。在Sprague Dawley大鼠模型中，额后颅骨缝在出生后10至20天之间融合。研究了用中间的硅橡胶片将额后颅骨缝与其下方的硬脑膜分离的效果。60只8日龄的幼鼠被分为四组，每组15只。A组作为未手术的对照组。B组为实验组，接受开颅手术、硬脑膜抬高，并在额后颅骨缝和下方硬脑膜之间插入硅橡胶片。包括两个手术假手术组。C组仅接受开颅手术和硬脑膜移位。D组仅接受开颅手术，不进行硬脑膜移位。在15、22和30日龄时处死大鼠。结果显示，未手术的动物（A组）在15天时显示出正常的缝线融合起始，并在22天时完全融合。B组动物放置了硅橡胶片屏障，在22天时缝线持续开放。缝线融合起始延迟至30天。假手术C组，即接受开颅手术和硬脑膜移位的动物，显示融合起始延迟至22天，在30日龄时完全融合。假手术D组，仅进行开颅手术，其缝线融合的时间顺序与未手术的对照组A相同。这些数据表明，当缝线与硬脑膜的相互作用被手术放置的屏障或简单的硬脑膜移位中断时，正常的颅骨缝融合会延迟。此外，硬脑膜与上方缝线之间的相互作用似乎指导着缝线融合。
