蚊子对登革2型病毒传播的基因工程抗性。
Genetically engineered resistance to dengue-2 virus transmission in mosquitoes.
作者信息
Olson K E, Higgs S, Gaines P J, Powers A M, Davis B S, Kamrud K I, Carlson J O, Blair C D, Beaty B J
机构信息
Arthropod-Borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
出版信息
Science. 1996 May 10;272(5263):884-6. doi: 10.1126/science.272.5263.884.
The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.
控制登革热等节肢动物传播的病毒疾病，最终可能需要对蚊媒进行基因操纵，以阻断病毒向人类群体的传播。为降低蚊子传播登革热病毒的能力，一种重组辛德毕斯病毒被用于转导埃及伊蚊雌蚊，该病毒携带一段针对2型登革热病毒（DEN-2）前膜编码区的567个碱基的反义RNA。被转导的蚊子无法在其唾液腺中支持DEN-2病毒的复制，因此无法传播该病毒。
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