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肺肿瘤细胞系中凋亡调控基因的表达：与p53表达的关系及与获得性耐药的相关性

Expression of apoptosis-regulatory genes in lung tumour cell lines: relationship to p53 expression and relevance to acquired drug resistance.

作者信息

Reeve J G, Xiong J, Morgan J, Bleehen N M

机构信息

Medical Research Council Clinical Oncology and Radiotherapeutics Unit, Medical Research Council Centre, Cambridge, UK.

出版信息

Br J Cancer. 1996 May;73(10):1193-200. doi: 10.1038/bjc.1996.230.

DOI:10.1038/bjc.1996.230
PMID:8630278
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2074502/
Abstract

As a first step towards elucidating the potential role(s) of bcl-2 and bcl-2-related genes in lung tumorigenesis and therapeutic responsiveness, the expression of these genes has been examined in a panel of lung cancer cell lines derived from untreated and treated patients, and in cell lines selected in vitro for multidrug resistance. Bcl-2 was hyperexpressed in 15 of 16 small-cell lung cancer (SCLC) cell lines and two of five non-small-cell lung cancer (NSCLC) lines compared with normal lung and brain, and hyperexpression was not chemotherapy related. Bcl-x was hyperexpressed in the majority of SCLC and NSCLC cell lines as compared with normal tissues, and all lung tumour lines preferentially expressed bcl-x1-mRNA, the splice variant form that inhibits apoptosis. Bax gene transcripts were hyperexpressed in most SCLC and NSCLC cell lines examined compared with normal adult tissues. Mutant p53 gene expression was detected in the majority of the cell lines and no relationship between p53 gene expression and the expression of either bcl-2, bcl-x or bax was observed. No changes in bcl-2, bcl-x and bax gene expression were observed in multidrug-resistant cell lines compared with their drug-sensitive counterparts.

摘要

作为阐明bcl-2及bcl-2相关基因在肺癌发生和治疗反应中潜在作用的第一步，已对一组来源于未经治疗和经治疗患者的肺癌细胞系，以及体外筛选出的多药耐药细胞系中这些基因的表达进行了检测。与正常肺组织和脑组织相比，16个小细胞肺癌（SCLC）细胞系中的15个以及5个非小细胞肺癌（NSCLC）细胞系中的2个bcl-2呈高表达，且高表达与化疗无关。与正常组织相比，大多数SCLC和NSCLC细胞系中bcl-x呈高表达，并且所有肺肿瘤细胞系均优先表达bcl-x1-mRNA，即抑制细胞凋亡的剪接变异体形式。与正常成人组织相比，在所检测的大多数SCLC和NSCLC细胞系中Bax基因转录本呈高表达。在大多数细胞系中检测到突变型p53基因表达，未观察到p53基因表达与bcl-2、bcl-x或bax表达之间存在关联。与药敏细胞系相比，多药耐药细胞系中bcl-2、bcl-x和bax基因表达未发生变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/07949ab76419/brjcancer00038-0041-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/58c4ee12c1d1/brjcancer00038-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/2039a3dcf8f3/brjcancer00038-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/a4d61db27390/brjcancer00038-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/548a9b192f35/brjcancer00038-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/07949ab76419/brjcancer00038-0041-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/58c4ee12c1d1/brjcancer00038-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/2039a3dcf8f3/brjcancer00038-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/a4d61db27390/brjcancer00038-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/548a9b192f35/brjcancer00038-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa4c/2074502/07949ab76419/brjcancer00038-0041-c.jpg

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