Chui X, Egami H, Yamashita J, Kurizaki T, Ohmachi H, Yamamoto S, Ogawa M
Department of Surgery II, Kumamoto University Medical School, Japan.
Br J Cancer. 1996 May;73(10):1233-6. doi: 10.1038/bjc.1996.236.
The immunohistochemical expression of c-kit proto-oncogene product in 57 breast cancer tissues was studied using anti-c-kit proto-oncogene product antibody in comparison with 20 normal breast tissues and 58 benign breast tumours. In normal breast tissues, the c-kit proto-oncogene product was strongly expressed on cell membrane and/or cytoplasm of alveolar and ductal cells. The immunoreactive score (IRS) of c-kit proto-oncogene product in normal mammary epithelia was 6.22 +/- 2.11 (mean +/- s.d.). In benign breast diseases, the c-kit proto-oncogene product was detected heterogeneously with a reduced IRS (3.33 +/- 2.44). In breast cancer tissues, the expression of the immunoreactive c-kit proto-oncogene product was often deleted and the average IRS was significantly reduced compared to those of normal breast tissues or benign breast diseases tissues. Among benign diseases, the average IRS of intraductal papilloma was significantly reduced (1.34 +/- 1.70) and the staining intensity and pattern were found to be similar to those seen in breast cancer. The results in this study suggested that the c-kit proto-oncogene product is correlated with the growth control or the differentiation of normal breast epithelium. Also, the loss of the expression of this protein may indicate the change of the signal transduction in relation to malignant transformation in human mammary epithelium.
采用抗c-kit原癌基因产物抗体，对57例乳腺癌组织中c-kit原癌基因产物的免疫组化表达进行研究，并与20例正常乳腺组织和58例乳腺良性肿瘤进行比较。在正常乳腺组织中，c-kit原癌基因产物在腺泡细胞和导管细胞的细胞膜和/或细胞质上呈强表达。正常乳腺上皮中c-kit原癌基因产物的免疫反应评分（IRS）为6.22±2.11（平均值±标准差）。在乳腺良性疾病中，c-kit原癌基因产物呈异质性检测，IRS降低（3.33±2.44）。在乳腺癌组织中，免疫反应性c-kit原癌基因产物的表达常缺失，与正常乳腺组织或乳腺良性疾病组织相比，平均IRS显著降低。在良性疾病中，导管内乳头状瘤的平均IRS显著降低（1.34±1.70），其染色强度和模式与乳腺癌相似。本研究结果表明，c-kit原癌基因产物与正常乳腺上皮的生长调控或分化相关。此外，该蛋白表达的缺失可能表明人乳腺上皮细胞恶性转化过程中信号转导的改变。
