Strohmeyer T, Peter S, Hartmann M, Munemitsu S, Ackermann R, Ullrich A, Slamon D J
Department of Medicine, University of California, School of Medicine, Los Angeles 90024.
Cancer Res. 1991 Apr 1;51(7):1811-6.
Seventy testicular germ cell tumors were analyzed at the DNA and RNA levels for the c-kit, hst-1, and int-2 oncogenes using Northern and Southern blot analyses, respectively. There were significant differences in oncogene expression between seminomas and nonseminomas with c-kit being expressed in 24 of 30 (80%) seminomas but in only 3 of 40 (7%) nonseminomatous tumors (P = 0.0001, chi 2 test) and hst-1 being expressed in 24 of 38 (63%) nonseminomas but only 1 of 24 (4%) of seminomas (P = 0.0001, chi 2 test), demonstrating an inverse relationship in the expression pattern of these 2 oncogenes in human testicular germ cell tumors. A significant association between tumor stage and hst-1 expression in the nonseminoma group was found (P = 0.0002, chi 2 test). No gross alterations in the c-kit, hst-1, and int-2 loci were found at the DNA level and no int-2 mRNA expression was detected in any of the germ cell tumors examined.
分别使用Northern印迹法和Southern印迹法，在DNA和RNA水平对70例睾丸生殖细胞肿瘤的c-kit、hst-1和int-2癌基因进行了分析。精原细胞瘤和非精原细胞瘤之间的癌基因表达存在显著差异，c-kit在30例精原细胞瘤中的24例（80%）表达，但在40例非精原细胞瘤中仅3例（7%）表达（P = 0.0001，卡方检验），hst-1在38例非精原细胞瘤中的24例（63%）表达，但在24例精原细胞瘤中仅1例（4%）表达（P = 0.0001，卡方检验），表明这两种癌基因在人类睾丸生殖细胞肿瘤中的表达模式呈负相关。在非精原细胞瘤组中发现肿瘤分期与hst-1表达之间存在显著关联（P = 0.0002，卡方检验）。在DNA水平未发现c-kit、hst-1和int-2基因座有明显改变，在所检测的任何生殖细胞肿瘤中均未检测到int-2 mRNA表达。
