通过一种新的细胞荧光测定法鉴定骨髓和血液样本中的白血病细胞。
Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
作者信息
Hengstschläger M, Pfeilstöcker M, Wawra E
机构信息
Institute of Molecular Biology, University of Vienna, Vienna Biocenter, Austria.
出版信息
Br J Cancer. 1996 May;73(10):1237-40. doi: 10.1038/bjc.1996.237.
Abstract
The expression of thymidine kinase--an enzyme of the DNA precursor pathway--is strictly regulated during the normal cellular cycle, but is much higher and permanently expressed in malignant growing cells. We used this fact to detect neoplastic cells in samples freshly taken from leukaemia patients and kept frozen in liquid nitrogen until analysis. Using a new cytofluorometric assay for thymidine kinase in single cells, we were able to identify leukaemic cells in a surplus of normal ones. Our results demonstrate the benefits of this assay for leukaemia diagnosis.
摘要
胸苷激酶是DNA前体途径中的一种酶，其表达在正常细胞周期中受到严格调控，但在恶性生长的细胞中表达水平更高且持续存在。我们利用这一事实，对从白血病患者新鲜采集并保存在液氮中直至分析的样本中的肿瘤细胞进行检测。通过一种针对单细胞胸苷激酶的新型细胞荧光分析方法，我们能够在大量正常细胞中识别出白血病细胞。我们的结果证明了该分析方法在白血病诊断中的优势。
相似文献
1
Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.通过一种新的细胞荧光测定法鉴定骨髓和血液样本中的白血病细胞。
Br J Cancer. 1996 May;73(10):1237-40. doi: 10.1038/bjc.1996.237.
2
Thymidine kinase expression. A marker for malignant cells.
Adv Exp Med Biol. 1998;431:455-60.
3
Cytofluorometric determination of thymidine kinase activity in a mixture of normal and neoplastic cells.正常细胞与肿瘤细胞混合物中胸苷激酶活性的细胞荧光测定法。
Br J Cancer. 1993 May;67(5):1022-5. doi: 10.1038/bjc.1993.187.
4
[Autoradiographic and enzyme-chemical studies of DNA synthesis in leukemia cells. I. Correlation of thymidine-kinase and 3 H-thymidine labelling index].白血病细胞中DNA合成的放射自显影和酶化学研究。I. 胸苷激酶与³H-胸苷标记指数的相关性
Klin Wochenschr. 1970 Jul 1;48(13):780-8. doi: 10.1007/BF01494493.
5
Thymidine kinase activity in the human bone marrow from various blood diseases.各种血液疾病患者骨髓中的胸苷激酶活性。
Life Sci. 1968 Apr 15;7(8):395-9. doi: 10.1016/0024-3205(68)90039-8.
6
Single-cell immunofluorescence assay for terminal transferase: human leukaemic and non-leukaemic cells.末端转移酶的单细胞免疫荧光测定：人类白血病细胞和非白血病细胞
Br J Cancer. 1980 Feb;41(2):159-67. doi: 10.1038/bjc.1980.26.
7
[Possible diagnostic value of the cytochemical staining of arylsulfatase in hematology. Preliminary data].
Nouv Rev Fr Hematol. 1969 Sep-Oct;9(5):706-10.
8
Reverse transcriptase activity: increase in marrow cultures from leukaemic patients in relapse and remission.逆转录酶活性：白血病复发和缓解患者骨髓培养物中的活性增加。
Br J Cancer. 1974 Jun;29(6):433-7. doi: 10.1038/bjc.1974.95.
9
Characterization of hematologic malignancies by flow cytometry.通过流式细胞术对血液系统恶性肿瘤进行特征分析。
Blood Cells. 1980;6(4):719-44.
10
Cytofluorometric assay for the determination of thymidine uptake and phosphorylation in living cells.
Cytometry. 1993;14(1):39-45. doi: 10.1002/cyto.990140108.
引用本文的文献
1
A new cytofluorometric approach to detect fetal cells in the maternal circulation.一种检测母血循环中胎儿细胞的新型细胞荧光分析方法。
J Med Genet. 1997 Sep;34(9):789-90. doi: 10.1136/jmg.34.9.789.
本文引用的文献
1
Hematopoietic cell proliferation and differentiation.
Curr Opin Hematol. 1994 Jul;1(4):310-20.
2
Loss of the p16/MTS1 tumor suppressor gene causes E2F-mediated deregulation of essential enzymes of the DNA precursor metabolism.
DNA Cell Biol. 1996 Jan;15(1):41-51. doi: 10.1089/dna.1996.15.41.
3
A binding site for transcription factor E2F is a target for trans activation of murine thymidine kinase by polyomavirus large T antigen and plays an important role in growth regulation of the gene.转录因子E2F的结合位点是多瘤病毒大T抗原对小鼠胸苷激酶进行反式激活的靶点，并且在该基因的生长调节中起重要作用。
J Virol. 1993 Apr;67(4):1765-71. doi: 10.1128/JVI.67.4.1765-1771.1993.
4
Cytofluorometric determination of thymidine kinase activity in a mixture of normal and neoplastic cells.正常细胞与肿瘤细胞混合物中胸苷激酶活性的细胞荧光测定法。
Br J Cancer. 1993 May;67(5):1022-5. doi: 10.1038/bjc.1993.187.
5
A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4.细胞周期调控中的一种新调控基序，可特异性抑制细胞周期蛋白D/细胞周期蛋白依赖性激酶4。
Nature. 1993 Dec 16;366(6456):704-7. doi: 10.1038/366704a0.
6
Different regulation of thymidine kinase during the cell cycle of normal versus DNA tumor virus-transformed cells.
J Biol Chem. 1994 May 13;269(19):13836-42.
7
Cell cycle. Stifled by inhibitions.
Nature. 1994 Sep 15;371(6494):204-5. doi: 10.1038/371204a0.
8
Purine and pyrimidine metabolism in human gliomas: relation to chromosomal aberrations.人类神经胶质瘤中的嘌呤和嘧啶代谢：与染色体畸变的关系。
Br J Cancer. 1994 Aug;70(2):212-8. doi: 10.1038/bjc.1994.282.
9
Tumour diagnosis.
Nature. 1994 Jun 30;369(6483):701. doi: 10.1038/369701c0.
10
The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA.细胞周期蛋白依赖性激酶的p21抑制剂通过与增殖细胞核抗原相互作用来控制DNA复制。
Nature. 1994 Jun 16;369(6481):574-8. doi: 10.1038/369574a0.
Zotero 插件