Noriega F G, Pennington J E, Barillas-Mury C, Wang X Y, Wells M A
Department of Biochemistry and Center for Insect Science, University of Arizona, Tucson, 85721, USA.
Insect Mol Biol. 1996 Feb;5(1):25-9. doi: 10.1111/j.1365-2583.1996.tb00037.x.
Early trypsin is a female-specific protease present in the Aedes aegypti midgut during the first hours after ingestion of a blood meal. Early trypsin gene expression was studied by Northern blot analysis. The early trypsin mRNA, absent in larvae, pupae and newly emerged females, reaches detectable levels at 24 h post-emergence and attains a maximum level at an adult age of 4-7 days. After the first week there is a decrease in the steady-state level of the transcript, but it remains readily detectable for up to a month after emergence. Despite the high levels of early trypsin mRNA present in the midgut of the unfed female, translation of the early trypsin mRNA occurs only after a blood or a protein meal. Early trypsin mRNA levels rapidly decrease during the first 24 h after feeding, but the steady-state level of the transcript rises again at the end of the blood digestion cycle (60 h), as the mosquito prepares for a second blood meal.
早期胰蛋白酶是一种雌性特异性蛋白酶，在埃及伊蚊摄取血餐之后的最初几个小时存在于其肠道中。通过Northern印迹分析研究了早期胰蛋白酶基因的表达。早期胰蛋白酶mRNA在幼虫、蛹和刚羽化的雌性中不存在，在羽化后24小时达到可检测水平，并在成虫4至7日龄时达到最高水平。第一周后，转录本的稳态水平下降，但在羽化后长达一个月内仍易于检测到。尽管未进食的雌性肠道中存在高水平的早期胰蛋白酶mRNA，但早期胰蛋白酶mRNA仅在摄入血餐或蛋白质餐之后才会发生翻译。喂食后的最初24小时内，早期胰蛋白酶mRNA水平迅速下降，但在血液消化周期结束时（60小时），随着蚊子为第二次血餐做准备，转录本的稳态水平再次上升。
