Department of Entomology, Texas A&M University, College Station, TX, USA.
Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.
Arch Virol. 2020 Aug;165(8):1769-1776. doi: 10.1007/s00705-020-04652-0. Epub 2020 May 21.
South Texas has experienced local transmission of Zika virus and of other mosquito-borne viruses such as chikungunya virus and dengue virus in the last decades. Using a mosquito surveillance program in the Lower Rio Grande Valley (LRGV) and San Antonio, TX, from 2016 to 2018, we detected the presence of an insect-specific virus, cell fusing agent virus (CFAV), in the Aedes aegypti mosquito population. We tested 6,326 females and 1,249 males from the LRGV and 659 females from San Antonio for CFAV by RT-PCR using specific primers. Infection rates varied from 0 to 261 per 1,000 mosquitoes in the LRGV and 115 to 208 per 1,000 in San Antonio depending on the month of collection. Infection rates per 1,000 individuals appeared higher in females collected from BG Sentinel 2 traps compared to Autocidal Gravid Ovitraps, but the ratio of the percentage of infected pools did not differ by trap type. The natural viral load in individual males ranged from 1.25 x 10 to 5.50 x 10 RNA copies and in unfed females from 5.42 x 10 to 8.70 x 10 RNA copies. Gravid females were found to harbor fewer viral particles than males and unfed females.
过去几十年，南德克萨斯州曾经历过寨卡病毒以及基孔肯雅热病毒和登革热病毒等其他蚊媒病毒的本地传播。我们使用 2016 年至 2018 年在里奥格兰德河谷（LRGV）和德克萨斯州圣安东尼奥的蚊虫监测计划，在埃及伊蚊种群中检测到一种昆虫特异性病毒，细胞融合剂病毒（CFAV）。我们使用特定的引物通过 RT-PCR 测试了来自 LRGV 的 6,326 只雌性和 1,249 只雄性以及来自圣安东尼奥的 659 只雌性蚊虫的 CFAV。根据收集月份的不同，LRGV 的感染率从每千只蚊子 0 到 261 不等，圣安东尼奥的感染率从每千只蚊子 115 到 208 不等。与自动诱卵器相比，从 BG Sentinel 2 陷阱中收集的雌性个体的每千个人体感染率似乎更高，但感染池的百分比比值不因陷阱类型而异。个体雄性的天然病毒载量范围为 1.25 x 10 到 5.50 x 10 RNA 拷贝，未进食的雌性的天然病毒载量范围为 5.42 x 10 到 8.70 x 10 RNA 拷贝。与雄性和未进食的雌性相比，妊娠雌性携带的病毒颗粒较少。
