Nonaka M, Nonaka R, Jordana M, Dolovich J
Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada.
Am J Respir Crit Care Med. 1996 May;153(5):1675-81. doi: 10.1164/ajrccm.153.5.8630619.
Epithelial cells potentially contribute to airways inflammation by antigen presentation and the production of proinflammatory cytokines. This study investigated the immunocytochemical localization of interleukin-8 (IL-8), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-1 receptor (IL-1R Type I), tumor necrosis factor-alpha receptor (TNF-alpha R; 55kD), and human leukocyte antigen-DR (HLA-DR) on epithelial cells obtained by nasal brushing from 10 patients with allergic rhinitis in season and 15 healthy, nonallergic subjects. Six of the 15 nonallergic asymptomatic subjects had macroscopic evidence of nasal mucosal inflammation, and their brushings contained more than 10% neutrophils ("subclinical inflammation"). In normal control subjects, 8 +/- 7.5% of epithelial cells stained for HLA-DR, approximately one quarter stained for IL-8 and GM-CSF, and about one third stained positive for IL-1R and TNF-alpha R. The findings in subjects with allergic rhinitis in season and with subclinical neutrophilia were similar, and the numbers of cells staining for HLA-DR expression correlated with both neutrophil and lymphocyte content. These findings further support the conclusion that epithelial cells can contribute to inflammatory processes in the nasal mucosa. The findings emphasize the need to identify asymptomatic nasal mucosal inflammation in studies of the nasal mucosa.
上皮细胞可能通过抗原呈递和促炎细胞因子的产生促进气道炎症。本研究调查了10例季节性变应性鼻炎患者和15名健康非变应性受试者经鼻刷检获得的上皮细胞上白细胞介素-8（IL-8）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-1受体（I型IL-1R）、肿瘤坏死因子-α受体（TNF-αR；55kD）和人类白细胞抗原-DR（HLA-DR）的免疫细胞化学定位。15名非变应性无症状受试者中有6名有鼻黏膜炎症的宏观证据，其刷检物中中性粒细胞含量超过10%（“亚临床炎症”）。在正常对照受试者中，8±7.5%的上皮细胞HLA-DR染色阳性，约四分之一的细胞IL-8和GM-CSF染色阳性，约三分之一的细胞IL-1R和TNF-αR染色阳性。季节性变应性鼻炎患者和亚临床中性粒细胞增多患者的结果相似，HLA-DR表达染色的细胞数量与中性粒细胞和淋巴细胞含量均相关。这些发现进一步支持上皮细胞可促进鼻黏膜炎症过程的结论。这些发现强调在鼻黏膜研究中识别无症状鼻黏膜炎症的必要性。
