Caballero Nadieska, Welch Kevin C, Carpenter Patrick S, Mehrotra Swati, O'Connell Tom F, Foecking Eileen M
Department of Otolaryngology - Head and Neck Surgery, Stritch School of Medicine, Pathology Department, Loyola University Medical Center, Maywood, Illinois, USA.
Allergy Rhinol (Providence). 2015 Jan;6(3):162-7. doi: 10.2500/ar.2015.6.0131.
Several population studies demonstrated an increased risk of allergic rhinitis in patients exposed to acetaminophen. However, no histologic studies have been conducted to assess the relationship between acetaminophen exposure and allergic rhinitis.
In this study, we investigated the association between chronic acetaminophen exposure and the development of allergic rhinitis in a rat model.
Ten female Sprague-Dawley rats were randomly assigned to either a control (n = 5) or an acetaminophen group (n = 5). The acetaminophen group received 200 mg/kg/day of acetaminophen suspended in yogurt via oral gavage for 120 days. The control group received only the yogurt vehicle. Allergic behavioral responses, including nose rub, eye rub, ear scratching, and neck and/or face scratching, were quantified. The rats were killed, and the noses were harvested. The portion of the nose, including the nasal septum and the inferior turbinates, was embedded in paraffin, sectioned, and stained with hematoxylin and eosin to quantify the inflammatory infiltrate.
The average number of allergic responses per animal was 13.2 in the acetaminophen group versus 6.2 in the control group (p = 0.032). All the rats in the acetaminophen group (100%) had mast cells infiltrating the lamina propria of the inferior turbinate, whereas mast cells were detected in only 40% of the animals in the control group. The average number of mast cells per animal in the acetaminophen group was 134 versus 21 in the control group (p = 0.048).
Our study was the first to demonstrate a histologic association between chronic exposure to acetaminophen and rhinitis. Further research to elucidate the mechanism that underlies these findings is necessary.
多项人群研究表明，接触对乙酰氨基酚的患者患过敏性鼻炎的风险增加。然而，尚未进行组织学研究来评估对乙酰氨基酚暴露与过敏性鼻炎之间的关系。
在本研究中，我们在大鼠模型中研究了慢性对乙酰氨基酚暴露与过敏性鼻炎发生之间的关联。
将10只雌性斯普拉格-道利大鼠随机分为对照组（n = 5）或对乙酰氨基酚组（n = 5）。对乙酰氨基酚组通过口服灌胃给予悬浮于酸奶中的200 mg/kg/天对乙酰氨基酚，持续120天。对照组仅给予酸奶载体。对包括蹭鼻、揉眼、抓耳以及抓挠颈部和/或面部在内的过敏行为反应进行量化。处死大鼠，采集鼻部。将包括鼻中隔和下鼻甲在内的鼻部组织包埋于石蜡中，切片，并用苏木精和伊红染色以量化炎症浸润。
对乙酰氨基酚组每只动物的平均过敏反应次数为13.2次，而对照组为6.2次（p = 0.032）。对乙酰氨基酚组的所有大鼠（100%）下鼻甲固有层有肥大细胞浸润，而对照组仅40%的动物检测到肥大细胞。对乙酰氨基酚组每只动物的肥大细胞平均数量为134个，而对照组为21个（p = 0.048）。
我们的研究首次证明了慢性暴露于对乙酰氨基酚与鼻炎之间的组织学关联。有必要进一步研究以阐明这些发现背后的机制。
