Saitoh S, Ikeda K, Koida I, Suzuki Y, Kobayashi M, Tsubota A, Arase Y, Chayama K, Murashima N, Kumada H
Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan.
Cancer. 1995 Oct 1;76(7):1139-44. doi: 10.1002/1097-0142(19951001)76:7<1139::aid-cncr2820760707>3.0.co;2-w.
Concanavalin A (Con A) affinity electrophoresis of serum alpha-fetoprotein (AFP) can distinguish hepatocellular carcinoma (HCC) from other malignancies when the serum AFP concentration is elevated. However, Con A has not been able to distinguish HCC from benign chronic liver disease such as cirrhosis or chronic hepatitis.
The Con A affinity electrophoresis of serum AFP was analyzed in patients with a serum AFP concentration greater than 50 ng/mL by antibody affinity electrophoresis and Western blotting in an attempt to distinguish hepatocellular carcinoma from benign chronic liver disease. Before the assay, the serum AFP concentrations were adjusted between 100 ng/ml and 300 ng/ml by concentrating or diluting the samples.
Of 180 patients with HCC, 44 (24%) had a single band and 91 (51%), 35 (19%), and 10 (6%) had 2, 3, and 4 bands, respectively. All 35 patients with chronic hepatitis had a single band. All but 1 of 72 patients with cirrhosis had a single band. Multiple AFP bands on Con A affinity electrophoresis appear to be diagnostic of HCC. This method has a sensitivity of 76%, a specificity of 99%, a positive predictive value of 99%, and a negative predictive value of 71% for detecting HCC. The number of AFP bands correlated with serum AFP concentration and tumor size in patients with HCC.
This assay is useful for distinguishing HCC from benign chronic liver diseases.
当血清甲胎蛋白（AFP）浓度升高时，血清甲胎蛋白的刀豆球蛋白A（Con A）亲和电泳可将肝细胞癌（HCC）与其他恶性肿瘤区分开来。然而，Con A一直无法将HCC与良性慢性肝病（如肝硬化或慢性肝炎）区分开来。
通过抗体亲和电泳和蛋白质印迹法，对血清AFP浓度大于50 ng/mL的患者进行血清AFP的Con A亲和电泳分析，试图将肝细胞癌与良性慢性肝病区分开来。在检测前，通过浓缩或稀释样本将血清AFP浓度调整至100 ng/ml至300 ng/ml之间。
180例HCC患者中，44例（24%）有一条带，91例（51%）、35例（19%）和10例（6%）分别有2条、3条和4条带。35例慢性肝炎患者均有一条带。72例肝硬化患者中，除1例外均有一条带。Con A亲和电泳上出现多条AFP带似乎可诊断为HCC。该方法检测HCC的灵敏度为76%，特异性为99%，阳性预测值为99%，阴性预测值为71%。HCC患者中AFP带的数量与血清AFP浓度和肿瘤大小相关。
该检测方法有助于将HCC与良性慢性肝病区分开来。
