Shimizu K, Taniichi T, Satomura S, Matsuura S, Taga H, Taketa K
Osaka Research Laboratory, Wako Pure Chemical Industries, Ltd., Hyogo, Japan.
Clin Chim Acta. 1993 Jan 31;214(1):3-12. doi: 10.1016/0009-8981(93)90297-h.
Diagnostic kits for determination of alpha-fetoprotein (AFP) carbohydrate chain microheterogeneity were developed using lectin affinity electrophoresis with Lens culinaris agglutinin-A (LCA-A) and erythro-agglutinating phytohemagglutinin-E4 (PHA-E4). Separated AFP bands by electrophoresis were detected with high sensitivity by antibody-affinity blotting and immunoenzymatic amplification. Densitometry was used to apportion lectin reactive AFPs. The within-run S.D. for proportions of AFP bands was below 3%. Band intensity was linearly related to AFP concentration between 2 and 200 ng/ml. Profiles of lectin reactive AFPs were compared in serum samples from 55 patients having liver diseases. The average values of lectin reactive AFPs for chronic hepatitis and liver cirrhosis patients were both below 13%, but those of hepatocellular carcinoma patients were above 25%. Correlation of data with disease states suggests that the methods can greatly facilitate the discrimination between benign and malignant liver diseases.
利用刀豆球蛋白 A（LCA-A）和红细胞凝集素植物血凝素-E4（PHA-E4）的凝集素亲和电泳技术，开发了用于测定甲胎蛋白（AFP）糖链微异质性的诊断试剂盒。通过抗体亲和印迹法和免疫酶扩增法，以高灵敏度检测电泳分离出的 AFP 条带。用密度测定法来区分凝集素反应性 AFP。AFP 条带比例的批内标准差低于 3%。条带强度与 2 至 200 ng/ml 之间的 AFP 浓度呈线性关系。比较了 55 例肝病患者血清样本中凝集素反应性 AFP 的图谱。慢性肝炎和肝硬化患者的凝集素反应性 AFP 平均值均低于 13%，而肝细胞癌患者的该平均值高于 25%。数据与疾病状态的相关性表明，这些方法能够极大地促进良性和恶性肝病的鉴别。
