Lam K Y, Chan A C, Chan K W, Leung M L, Srivastava G
Department of Pathology, Queen Mary Hospital, Hong Kong.
Eur J Surg Oncol. 1995 Dec;21(6):613-6. doi: 10.1016/s0748-7983(95)95262-4.
We studied p53 over-expression in a series of 42 primary penile carcinomas (seven verrucous carcinomas, 14 well-differentiated, 15 moderately-differentiated and six poorly-differentiated squamous cell carcinomas) from Chinese patients using the p53 protein specific mouse monoclonal antibody DO-7 on paraffin sections. p53 protein was detected in 40% (17 cases) of the tumours. The p53 staining was not observed in the penile warts (n = 6) and verrucous carcinomas (n = 7). Positive p53 staining was identified only in the less differentiated tumour cells in the periphery of the tumour cell nests in all the cases. The non-invasive dysplastic epithelium next to the tumours could also be positive for p53 protein (three out of 10 cases in which the dysplastic epithelium adjacent to the tumour was adequately sampled). Furthermore, 100% of the human papillomavirus (HPV)-positive cases showed positive p53 staining. It is concluded that p53 over-expression is present in penile squamous cell carcinomas and adjacent non-invasive tumour cells. An inverse correlation between HPV and p53 gene mutation is not observed in penile cancers.
我们使用p53蛋白特异性小鼠单克隆抗体DO-7对来自中国患者的42例原发性阴茎癌（7例疣状癌、14例高分化、15例中分化和6例低分化鳞状细胞癌）石蜡切片进行p53过表达研究。在40%（17例）的肿瘤中检测到p53蛋白。在阴茎疣（n = 6）和疣状癌（n = 7）中未观察到p53染色。在所有病例中，仅在肿瘤细胞巢周边分化较差的肿瘤细胞中发现p53染色阳性。肿瘤旁的非侵袭性发育异常上皮也可能p53蛋白呈阳性（在10例对肿瘤旁发育异常上皮进行充分取样的病例中有3例）。此外，100%的人乳头瘤病毒（HPV）阳性病例p53染色呈阳性。结论是，p53过表达存在于阴茎鳞状细胞癌及相邻的非侵袭性肿瘤细胞中。在阴茎癌中未观察到HPV与p53基因突变之间存在负相关。
