Margolin W, Wang R, Kumar M
Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston 77030, USA.
J Bacteriol. 1996 Mar;178(5):1320-7. doi: 10.1128/jb.178.5.1320-1327.1996.
We have isolated a homolog of the cell division gene ftsZ from the extremely halophilic archaebacterium Halobacterium salinarium. The predicted protein of 39 kDa is divergent relative to eubacterial homologs, with 32% identity to Escherichia coli FtsZ. No other eubacterial cell division gene homologs were found adjacent to H. salinarium ftsZ. Expression of the ftsZ gene region in H. salinarium induced significant morphological changes leading to the loss of rod shape. Phylogenetic analysis demonstrated that the H. salinarium FtsZ protein is more related to tubulins than are the FtsZ proteins of eubacteria, supporting the hypothesis that FtsZ may have evolved into eukaryotic tubulin.
我们从极端嗜盐古细菌盐生盐杆菌中分离出了细胞分裂基因ftsZ的一个同源物。预测的39 kDa蛋白质与真细菌同源物不同，与大肠杆菌FtsZ的同一性为32%。在盐生盐杆菌ftsZ附近未发现其他真细菌细胞分裂基因同源物。盐生盐杆菌中ftsZ基因区域的表达引起了显著的形态变化，导致杆状形态丧失。系统发育分析表明，盐生盐杆菌FtsZ蛋白比真细菌的FtsZ蛋白与微管蛋白的关系更密切，这支持了FtsZ可能已进化为真核微管蛋白的假说。
