Ueyama Y, Yokoyama M, Kondo Y, Ohsawa N, Tamoki N
Gan. 1977 Feb;68(1):121-3.
Five transplantable tumors, which originated from human maligant neoplasms and had been maintained in nude mice, were frozen at--70 degrees preserved for 1226 days. Tumor tissue was minced in a mixture of tissue culture medium, 10% dimethyl sulfoxide, and 10% calf serum before freezing. The transplantability of frozen tumors was examined after thawing. Morphological and functional characteristic of each tumor were compared before and after the frozen storage. The results suggest that low-temperature preservation technique can be applied satisfactorily for the maintenance of human malignant tumors which have been maintained by using nude mice.
五种源自人类恶性肿瘤并在裸鼠体内传代的可移植肿瘤，在-70℃下冷冻保存1226天。冷冻前，将肿瘤组织在含有组织培养基、10%二甲基亚砜和10%小牛血清的混合液中切碎。解冻后检测冷冻肿瘤的可移植性。比较冷冻保存前后各肿瘤的形态学和功能特征。结果表明，低温保存技术可成功应用于维持通过裸鼠传代的人类恶性肿瘤。
