子痫前期患者人血管内皮细胞钙依赖性一氧化氮的产生
The calcium-dependent nitric oxide production of human vascular endothelial cells in preeclampsia.
作者信息
Orpana A K, Avela K, Ranta V, Viinikka L, Ylikorkala O
机构信息
Department of Obstetrics and Gynecology, University of Helsinki, Finland.
出版信息
Am J Obstet Gynecol. 1996 Mar;174(3):1056-60. doi: 10.1016/s0002-9378(96)70350-2.
OBJECTIVE
Nitric oxide is an important vasodilator, and in this study we studied whether the calcium-dependent nitric oxide production capacity of human umbilical vein endothelial cells was affected by preeclampsia.
STUDY DESIGN
Human umbilical vein endothelial cells were isolated from 11 preeclamptic and 10 normotensive pregnancies. The maximal calcium ionophore A23187-stimulated nitric oxide production capacity was measured as accumulation of nitrate and nitrite into the culture medium, and it was related to the number of viable endothelial cells by measurement of their mitochondrial dehydrogenase activity.
RESULTS
The cell number-related nitric oxide production capacity was similar in preeclamptic and normotensive pregnancies. The total nitric oxide production of cells from preeclamptic pregnancies was significantly lower (p <0.001). This difference, however, was mainly caused by larger amount of viable endothelial cells recovered from normotensive pregnancies.
CONCLUSION
The maximal calcium-dependent nitric oxide production capacity of individual human umbilical vein endothelial cells is not affected by preeclampsia.
目的
一氧化氮是一种重要的血管舒张剂，在本研究中，我们研究了子痫前期是否会影响人脐静脉内皮细胞中依赖钙的一氧化氮生成能力。
研究设计
从11例子痫前期孕妇和10例血压正常孕妇中分离出人脐静脉内皮细胞。通过测量培养基中硝酸盐和亚硝酸盐的积累来测定最大钙离子载体A23187刺激的一氧化氮生成能力，并通过测量其线粒体脱氢酶活性将其与存活的内皮细胞数量相关联。
结果
子痫前期孕妇和血压正常孕妇中与细胞数量相关的一氧化氮生成能力相似。子痫前期孕妇细胞的一氧化氮总生成量显著较低（p<0.001）。然而，这种差异主要是由血压正常孕妇中回收的存活内皮细胞数量较多所致。
结论
子痫前期不影响单个脐静脉内皮细胞的最大钙依赖性一氧化氮生成能力。
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