人脐静脉内皮细胞与子痫前期患者血清孵育后血管活性物质的产生
Production of vasoactive substances by human umbilical vein endothelial cells after incubation with serum from preeclamptic patients.
作者信息
Scalera F, Schlembach D, Beinder E
机构信息
Department of Obstetrics and Gynecology, University of Erlangen/Nuremberg, Universitaetsstr. 2-23, 91054, Erlangen, Germany.
出版信息
Eur J Obstet Gynecol Reprod Biol. 2001 Dec 1;99(2):172-8. doi: 10.1016/s0301-2115(01)00412-2.
OBJECTIVE
To determine the in vitro effect of serum from preeclamptic patients on the proliferation, viability and secretion of vasoactive substances by human umbilical vein endothelial cells (HUVEC).
STUDY DESIGN
HUVEC were incubated for 24h with sera from 16 preeclamptic, 19 healthy pregnant and 8 healthy nonpregnant women. Proliferation rates were determined by cell counting and vitality by trypan blue staining. The vasoactive substances, 6-keto-prostaglandin F(1alpha), nitrite and nitrate, and endothelin-1 (ET-1) were measured in endothelial cell supernatants.
RESULTS
The preeclamptic serum had no effect on cell proliferation or vitality compared with control sera. It induced more HUVEC production of ET-1, but not of prostacyclin (PGI2) or nitric oxide compared with control serum.
CONCLUSION
Preeclamptic serum appears to contain a factor(s) that specifically stimulates ET-1 secretion from HUVEC without altering cell growth or vitality.
目的
确定子痫前期患者血清对人脐静脉内皮细胞（HUVEC）增殖、活力及血管活性物质分泌的体外作用。
研究设计
将HUVEC分别与16例子痫前期患者、19例健康孕妇及8例健康非孕女性的血清孵育24小时。通过细胞计数确定增殖率，用台盼蓝染色确定活力。在内皮细胞上清液中检测血管活性物质6-酮-前列腺素F（1α）、亚硝酸盐和硝酸盐以及内皮素-1（ET-1）。
结果
与对照血清相比，子痫前期血清对细胞增殖或活力无影响。与对照血清相比，它诱导HUVEC产生更多的ET-1，但不影响前列环素（PGI2）或一氧化氮的产生。
结论
子痫前期血清似乎含有一种因子，可特异性刺激HUVEC分泌ET-1，而不改变细胞生长或活力。
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