Brockman A H, Orlando R
Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
Anal Chem. 1995 Dec 15;67(24):4581-5. doi: 10.1021/ac00120a024.
A new technique has been developed that provides affinity separations directly on the surface of a matrix-assisted laser desorption/ionization mass spectrometer (MALDI-MS) probe. This strategy provides both in situ identification of biomolecules through biospecific antibody/antigen interactions and molecular weight information in a rapid and facile manner. Our technique, which we call probe affinity MS, directly couples the high selectivity of immobilized affinity chromatography with the sensitivity of MALDI-MS. As this technique permits the rapid identification of antigens, ligands, and other compounds from complex biological mixtures, we believe that it will be useful in a wide range of applications in virtually all fields of the life sciences.
一种新技术已经被开发出来，它能直接在基质辅助激光解吸/电离质谱仪（MALDI-MS）探针表面进行亲和分离。这种策略通过生物特异性抗体/抗原相互作用以快速简便的方式提供生物分子的原位鉴定和分子量信息。我们的技术，我们称之为探针亲和质谱，将固定化亲和色谱的高选择性与MALDI-MS的灵敏度直接结合起来。由于这项技术能够从复杂的生物混合物中快速鉴定抗原、配体和其他化合物，我们相信它将在生命科学几乎所有领域的广泛应用中发挥作用。
