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2
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A cyanobacterium capable of swimming motility.一种能够进行游动运动的蓝细菌。
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An unusual phycoerythrin from a marine cyanobacterium.一种来自海洋蓝藻的不寻常藻红蛋白。
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Novel Phycoerythrins in Marine Synechococcus spp. : Characterization and Evolutionary and Ecological Implications.海洋聚球藻属中的新型藻红蛋白：特性及其进化和生态意义
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Isolation and Molecular Characterization of Five Marine Cyanophages Propagated on Synechococcus sp. Strain WH7803.海洋蓝藻噬菌体的分离及其分子特征分析——以 WH7803 聚球藻为宿主
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Resistance to co-occurring phages enables marine synechococcus communities to coexist with cyanophages abundant in seawater.对共现噬菌体的抗性使海洋聚球藻群落能够与海水中丰富的噬藻体共存。
Appl Environ Microbiol. 1993 Oct;59(10):3393-9. doi: 10.1128/aem.59.10.3393-3399.1993.
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Chemotaxis toward Nitrogenous Compounds by Swimming Strains of Marine Synechococcus spp.海洋聚球藻属游动菌株对含氮化合物的趋化性
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Rod structure of a phycoerythrin II-containing phycobilisome. II. Complete sequence and bilin attachment site of a phycoerythrin gamma subunit.含藻红蛋白II的藻胆体的杆状结构。II. 藻红蛋白γ亚基的完整序列及胆色素附着位点
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Rod structure of a phycoerythrin II-containing phycobilisome. I. Organization and sequence of the gene cluster encoding the major phycobiliprotein rod components in the genome of marine Synechococcus sp. WH8020.含藻红蛋白II的藻胆体的杆状结构。I. 编码海洋聚球藻属菌株WH8020基因组中主要藻胆蛋白杆状成分的基因簇的组织和序列。
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9
Insertional inactivation of genes to isolate mutants of Synechococcus sp. strain PCC 7942: isolation of filamentous strains.通过基因插入失活分离聚球藻属PCC 7942菌株的突变体：丝状菌株的分离
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The response of the picoplanktonic marine cyanobacterium Synechococcus species WH7803 to phosphate starvation involves a protein homologous to the periplasmic phosphate-binding protein of Escherichia coli.微微型海洋蓝细菌聚球藻属物种WH7803对磷酸盐饥饿的响应涉及一种与大肠杆菌周质磷酸盐结合蛋白同源的蛋白质。
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一种用于聚球藻属海洋蓝细菌的基因操作系统。

A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus.

作者信息

Brahamsha B

机构信息

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202, USA.

出版信息

Appl Environ Microbiol. 1996 May;62(5):1747-51. doi: 10.1128/aem.62.5.1747-1751.1996.

DOI:10.1128/aem.62.5.1747-1751.1996
PMID:8633873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC167949/
Abstract

Unicellular cyanobacteria of the genus Synechococcus are among the most abundant members of the picoplankton in the open ocean, and their contribution to primary production is considerable. While several isolates have been used for physiological, biochemical, and molecular studies of their unique adaptations to the marine environment, it has become necessary to develop molecular genetic methods for one or more model open-ocean cyanobacteria in order for studies of these organisms and their unique properties to progress. A number of molecular tools for the genetic manipulation of Synechococcus sp. strains WH7803, WH8102, and WH8103 have been developed. These include a plating technique for obtaining isolated colonies at high efficiencies and a conjugation method for introducing both a replicative vector and a suicide vector. In addition, a method for the generation of random, tagged chromosomal insertions (N. Dolganov and A. R. Grossman, J. Bacteriol. 175:7644-7651, 1993; N. F. Tsinoremas, A. K. Kutach, C. A. Strayer, and S. S. Golden, J. Bacteriol. 176:6764-6768, 1994) has been applied to these organisms.

摘要

聚球藻属的单细胞蓝细菌是开阔海洋中微微型浮游生物中数量最多的成员之一，它们对初级生产的贡献相当可观。虽然已有几种分离菌株被用于对其独特适应海洋环境的生理、生化和分子研究，但为了这些生物及其独特特性的研究取得进展，开发针对一种或多种开阔海洋蓝细菌模式菌株的分子遗传学方法已变得十分必要。现已开发出多种用于聚球藻属菌株WH7803、WH8102和WH8103基因操作的分子工具。这些工具包括一种高效获得分离菌落的平板培养技术和一种用于导入复制型载体和自杀型载体的接合方法。此外，一种产生随机标记染色体插入的方法（N. 多尔加诺夫和A. R. 格罗斯曼，《细菌学杂志》175:7644 - 7651, 1993；N. F. 齐诺雷马斯、A. K. 库塔奇、C. A. 斯特雷耶和S. S. 戈尔登，《细菌学杂志》176:6764 - 6768, 1994）已应用于这些生物。