Thies S D, Corbin R S, Goff C D, Binns O A, Buchanan S A, Shockey K S, Frierson H F, Young J S, Tribble C G, Kron I L
Department of Surgery, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Ann Thorac Surg. 1996 May;61(5):1453-7. doi: 10.1016/0003-4975(96)00077-x.
Adult respiratory distress syndrome remains a major cause of morbidity and mortality. We investigated the role of thromboxane receptor antagonism in an experimental model of acute lung injury that mimics adult respiratory distress syndrome.
Three groups of rabbit heart-lung preparations were studied for 30 minutes in an ex vivo blood perfusion/ventilation system. Saline control (SC) lungs received saline solution during the first 20 minutes of study. Injury control (IC) lungs received an oleic acid-ethanol solution during the first 20 minutes. Thromboxane receptor blockade (TRB) lungs received the same injury as IC lungs, but a thromboxane receptor antagonist (SQ30741) was added to the blood perfusate just prior to study. Blood gases were obtained at 10-minute intervals, and tidal volume, pulmonary artery pressure, and lung weight were continuously recorded. Oxygenation was assessed by measuring the percent change in oxygen tension over the 30-minute study period. Tissue samples were collected from all lungs for histologic evaluation.
Significant differences were found between SC and IC lungs as well as TRB and IC lungs when comparing pulmonary artery pressure (SC = 33.1 +/- 2.2 mm Hg, TRB = 35.4 +/- 2.1 mm Hg, IC = 60.4 +/- 11.1 mm Hg; p < 0.02) and percent change in oxygenation (SC = -20.6% +/- 10.3%, TRB = -24.2% +/- 9.5%, IC = -57.1% +/- 6.2%; p < 0.03). None of the other variables demonstrated significant differences.
Thromboxane receptor blockade prevents the pulmonary hypertension and the decline in oxygenation seen in an experimental model of acute lung injury that mimics adult respiratory distress syndrome.
成人呼吸窘迫综合征仍然是发病和死亡的主要原因。我们在一个模拟成人呼吸窘迫综合征的急性肺损伤实验模型中研究了血栓素受体拮抗剂的作用。
在体外血液灌注/通气系统中对三组兔心肺标本进行了30分钟的研究。生理盐水对照组（SC）的肺在研究的前20分钟接受生理盐水溶液。损伤对照组（IC）的肺在最初20分钟接受油酸 - 乙醇溶液。血栓素受体阻断组（TRB）的肺接受与IC组相同的损伤，但在研究前将血栓素受体拮抗剂（SQ30741）加入血液灌注液中。每隔10分钟采集血气，连续记录潮气量、肺动脉压和肺重量。通过测量30分钟研究期间氧分压的变化百分比来评估氧合情况。从所有肺中采集组织样本进行组织学评估。
比较肺动脉压（SC = 33.1±2.2 mmHg，TRB = 35.4±2.1 mmHg，IC = 60.4±11.1 mmHg；p < 0.02）和氧合变化百分比（SC = -20.6%±10.3%，TRB = -24.2%±9.5%，IC = -57.1%±6.2%；p < 0.03）时，发现SC组与IC组以及TRB组与IC组之间存在显著差异。其他变量均未显示出显著差异。
在模拟成人呼吸窘迫综合征的急性肺损伤实验模型中，血栓素受体阻断可预防肺动脉高压和氧合下降。
