Oeltgen P R, Horton N D, Bolling S F, Su T P
Department of Pathology and Laboratory Medicine Service, College of Medicine, University of Kentucky, Lexington 40536, USA.
Ann Thorac Surg. 1996 May;61(5):1488-93. doi: 10.1016/0003-4975(96)00108-7.
The complications of preserving lungs for transplantation are well known, with successful transplantation only being assured by preservation times of 5 to 6 hours or less. If a new method of consistent lung preservation could be identified, lung transplantation could be extended to many patients. We have previously reported lung preservation times averaging 14.8 hours using a multiorgan autoperfusion block infused with physiologic saline solution as a model. When plasma from deeply hibernating woodchucks (Marmota monax) or the delta opioid DADLE was infused into the multi-organ block, lung preservation times increased threefold to 45 hours.
In this study, we examined the effect of infusing plasma containing the hibernation induction trigger molecule on lung preservation for transplantation using a multiorgan autoperfusion block.
This study demonstrated that successful orthotopic transplantation of single canine lungs is possible after 24 to 33 hours of preservation when the lung has been maintained with plasma containing the hibernation induction trigger molecule.
Theoretically, hibernation induction trigger could be administered to donors before lung harvest in an effort to extend lung preservation times.
肺移植供肺保存的并发症是众所周知的，只有在保存时间为5至6小时或更短的情况下才能确保移植成功。如果能找到一种持续肺保存的新方法，肺移植的适用患者范围就能扩大。我们之前曾报道，以灌注生理盐水溶液的多器官自体灌注模块为模型，肺保存时间平均为14.8小时。当将深度冬眠土拨鼠（Marmota monax）的血浆或δ阿片肽DADLE注入多器官模块时，肺保存时间增加了两倍，达到45小时。
在本研究中，我们使用多器官自体灌注模块，研究了注入含有冬眠诱导触发分子的血浆对肺移植保存的影响。
本研究表明，当用含有冬眠诱导触发分子的血浆保存肺时，单只犬肺在保存24至33小时后成功进行原位移植是可能的。
从理论上讲，可以在供体取肺前给予其冬眠诱导触发分子，以延长肺的保存时间。
