Wang Xiu-Hong, Zeng Jian-Feng, Lin Chao, Chen Shi-Biao
Department of Anesthesiology, Jiangxi Provincial People's Hospital Nanchang, China.
Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15692-9. eCollection 2015.
This study aims to explore the treatment method of myocardial ischemia-reperfusion injury.
Myocardial Ischemia-reperfusion rabbit model was established in this study. They were divided into four groups: sham operation (S) group, IRI control (I/R) group and IRI with morphine (MF) group and sufentanil (SF). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis.
Myocardial infarct size of control group and morphine and sufetanil group was 36.0±3.6, 23.0±1.2 and 27.1±2.3, respectively. There were significant differences between them (P < 0.01). Apoptotic index of I/R, MF and SF groups was 26.9±2.2, 12.5±2.3, 15.8±2.0, with statistical significance (P < 0.05). The concentration of CK-MB in serum: there were no significant differences of CK-MB between each group at baseline. The concentration of CK-MB after reperfusion were higher than that of baseline, except for group S (P < 0.05); Compared with group S, after reperfusion, the CK-MB of other three groups were higher (P < 0.05); The concentration of CK-MB in group MF and SF were lower than group I/R (P < 0.05); In contrast to group MF, the concentration of CK-MB after reperfusion was higher in group SF (P < 0.05).
Morphine and sufentanil can specifically protect the myocardial function.
本研究旨在探讨心肌缺血再灌注损伤的治疗方法。
本研究建立了心肌缺血再灌注兔模型。将它们分为四组：假手术（S）组、缺血再灌注对照（I/R）组、缺血再灌注加吗啡（MF）组和舒芬太尼（SF）组。采用苏木精-伊红（HE）染色法比较心肌梗死面积。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记（TUNEL）法检测细胞凋亡。
对照组、吗啡组和舒芬太尼组的心肌梗死面积分别为36.0±3.6、23.0±1.2和27.1±2.3。它们之间存在显著差异（P<0.01）。I/R组、MF组和SF组的凋亡指数分别为26.9±2.2、12.5±2.3、15.8±2.0，具有统计学意义（P<0.05）。血清肌酸激酶同工酶（CK-MB）浓度：各组基线时CK-MB无显著差异。再灌注后除S组外，其他各组CK-MB浓度均高于基线（P<0.05）；与S组相比，再灌注后其他三组的CK-MB更高（P<0.05）；MF组和SF组的CK-MB浓度低于I/R组（P<0.05）；与MF组相比，SF组再灌注后CK-MB浓度更高（P<0.05）。
吗啡和舒芬太尼可特异性保护心肌功能。
