Hicks M J, Flaitz C M, Westerman G H, Blankenau R J, Powell G L, Berg J H
Department of Pathology, Texas Children's Hospital, Houston 77030-2399, USA.
J Clin Pediatr Dent. 1995 Fall;20(1):9-13.
The aim of this study was to evaluate the effect of low fluence argon laser (AL) and acidulated phosphate fluoride (APF) treatment on caries initiation (CI) and progression (CP) in human enamel. Twenty caries-free molars were divided into tooth quarters. Tooth quarters from each specimen were assigned to one of four groups: 1) Control; 2) AL Only; 3) AL before APF; 4) APF before AL. AL was at 0.25 watts for 10 seconds (12.0 +/- 0.5 J/cm2). APF treatment was with a 1.23% gel for 4 minutes. Lesions were created in two treated, sound enamel windows per tooth quarter with an acidified gel. After CI and CP, sections were obtained and imbibed with water for polarized light study. Mean body of the lesion (BL) depths were determined and compared among groups (ANOVA & DMR). After CI, BL depths were: 189 +/- 29 micrometers for Control, 133 +/- 23 micrometers for AL only; 91 +/- 17 micrometers for AL before APF; and 83 +/- 14 micrometers for APF before AL. After CP, BL depths were: 321 +/- 43 micrometers for Control, 206 +/- 35 micrometers for AL only; 118 +/- 21 micrometers for AL before APF; and 114 +/- 19 micrometers for APF before AL. After CI and CP, argon laser irradiation alone resulted in significant reductions in lesion depth when compared with controls (p<0.05). APF treatment before or after argon laser exposure resulted in a significant reduction in lesion depth when compared with AL alone or control groups (p<0.05). Caries initiation and progression in vitro are affected to a significant extent when low fluence (energy) argon laser irradiation of sound enamel alone or in conjunction with APF treatment is done. This caries-protective effect occurs at an argon laser fluence (energy) that is capable of polymerizing visible light-cured resins.
本研究的目的是评估低能量氩激光（AL）和酸化磷酸氟（APF）处理对人牙釉质龋病起始（CI）和进展（CP）的影响。将20颗无龋磨牙分成牙体四分之一部分。每个样本的牙体四分之一部分被分配到四组之一：1）对照组；2）仅用AL组；3）APF前用AL组；4）AL前用APF组。AL能量为0.25瓦，照射10秒（12.0±0.5 J/cm2）。APF处理使用1.23%的凝胶，处理4分钟。在每个牙体四分之一部分的两个经过处理的完好牙釉质窗口中，用酸化凝胶制造龋损。在CI和CP之后，获取切片并用水浸泡以进行偏光研究。测定病变平均体部（BL）深度并在组间进行比较（方差分析和DMR）。CI之后，BL深度分别为：对照组189±29微米，仅用AL组133±23微米，APF前用AL组91±17微米，AL前用APF组83±14微米。CP之后，BL深度分别为：对照组321±43微米，仅用AL组206±35微米，APF前用AL组118±21微米，AL前用APF组114±19微米。CI和CP之后，与对照组相比，单独氩激光照射导致病变深度显著降低（p<0.05）。与单独使用AL组或对照组相比，在氩激光照射之前或之后进行APF处理导致病变深度显著降低（p<0.05）。当单独对完好牙釉质进行低能量（低通量）氩激光照射或与APF处理联合进行时，体外龋病的起始和进展受到显著影响。这种防龋作用发生在能够使可见光固化树脂聚合的氩激光能量水平上。
