鲤鱼丝裂原活化蛋白激酶激酶1基因的基因组结构

Genomic structure of carp mitogen-activated protein kinase kinase 1 gene.

作者信息

Leu J H, Lee M S, Chen K T, Chang G D, Chou C k, Huang C J

机构信息

Institute of Biological Chemistry, Academia Sinicia, Taipei, Taiwan.

出版信息

Biochim Biophys Acta. 1996 May 2;1306(2-3):133-6. doi: 10.1016/0167-4781(96)00023-1.

DOI:10.1016/0167-4781(96)00023-1

PMID:8634328

Abstract

Carp mitogen-activated protein kinase kinase 1 (cMKK1) gene was isolated from a liver genomic library. The sequence around the exon-intron boundaries and 2 kb of the promoter region were determined. Our data indicate that this gene is composed of 11 exons and 10 introns spanning about 9 kb. Multiple potential transcription initiation sites were located by primer extension analysis. Examination of 2 kb of 5'-flanking sequence revealed potential binding sites for a variety of transcription factors such as E2F, Ets-1, GATA-1, Myb, NF-IL6, Sp1, and NF-kB.

摘要

鲤鱼丝裂原活化蛋白激酶激酶1（cMKK1）基因是从肝脏基因组文库中分离出来的。确定了外显子-内含子边界周围的序列以及2 kb的启动子区域。我们的数据表明，该基因由11个外显子和10个内含子组成，跨越约9 kb。通过引物延伸分析确定了多个潜在的转录起始位点。对2 kb的5'侧翼序列进行检查，发现了多种转录因子（如E2F、Ets-1、GATA-1、Myb、NF-IL6、Sp1和NF-κB）的潜在结合位点。