Tooley P W, Garfinkel D J
USDA-ARS, Foreign Disease-Weed Science Research, Ft. Detrick, Frederick, MD 21701, USA.
Mol Plant Microbe Interact. 1996 May;9(4):305-9. doi: 10.1094/mpmi-9-0305.
Multiple copies of retrotransposon reverse transcriptase coding sequences were identified in Phytophthora infestans by polymerase chain reaction (PCR) amplification using degenerate primers. The P. infestans sequences belong to the Ty1-copia superfamily, and putative elements from different P. infestans isolates show restriction site polymorphisms. Some contain complete open reading frames while others do not, indicating the presence of potentially active as well as inactive elements.
利用简并引物通过聚合酶链反应（PCR）扩增，在致病疫霉中鉴定出多个反转录转座子逆转录酶编码序列的拷贝。致病疫霉的序列属于Ty1-copia超家族，不同致病疫霉分离株的推定元件显示出限制性位点多态性。一些含有完整的开放阅读框，而另一些则没有，这表明存在潜在的活性元件和非活性元件。
